All lanes : Anti-GRB2 antibody (ab79876) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell LysateLane 4 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell LysateLane 5 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell LysateLane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.