Anti-GRK2 antibody [Y137]

• Supplier: Abcam
• Catalog: ab32558
• Prices: $400.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: Y137
• Applications: WB IHC-P ICC/IF FC IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: A synthetic peptide corresponding to residues in the C-terminus of human GRK2
• Description: Rabbit Monoclonal
• Gene: ADRBK1
• Conjugate: Unconjugated

Product images

Anti-GRK2 antibody [Y137] (ab32558) at 1/500 dilution + U937 cell lysate

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab32558 at a dilution of 1/100 - 1/250

Overlay histogram showing HEK293 cells stained with ab32558 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32558, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) ( 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol/permeabilized in 0.1% PBS-Tween used under the same conditions.

Product References

Effects of the dopamine D2 allosteric modulator, PAOPA, on the expression of - Effects of the dopamine D2 allosteric modulator, PAOPA, on the expression of

Basu D, Tian Y, Bhandari J, Jiang JR, Hui P, Johnson RL, Mishra RK. PLoS One. 2013 Aug 6;8(8):e70736.

Migration of Th1 lymphocytes is regulated by CD152 (CTLA-4)-mediated signaling - Migration of Th1 lymphocytes is regulated by CD152 (CTLA-4)-mediated signaling

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Normalization of GRK2 protein and mRNA measures in patients with depression - Normalization of GRK2 protein and mRNA measures in patients with depression

Matuzany-Ruban A, Golan M, Miroshnik N, Schreiber G, Avissar S. Int J Neuropsychopharmacol. 2010 Feb;13(1):83-91.

Interleukin-33 attenuates sepsis by enhancing neutrophil influx to the site of - Interleukin-33 attenuates sepsis by enhancing neutrophil influx to the site of

Alves-Filho JC, Sonego F, Souto FO, Freitas A, Verri WA Jr, Auxiliadora-Martins M, Basile-Filho A, McKenzie AN, Xu D, Cunha FQ, Liew FY. Nat Med. 2010 Jun;16(6):708-12.

Pasteurella multocida toxin activates Gbetagamma dimers of heterotrimeric G - Pasteurella multocida toxin activates Gbetagamma dimers of heterotrimeric G

Preuss I, Kurig B, Nurnberg B, Orth JH, Aktories K. Cell Signal. 2009 Apr;21(4):551-8.