ADRBK1

Gene Symbol ADRBK1
Entrez Gene 156
Alt Symbol BARK1, BETA-ARK1, GRK2
Species Human
Gene Type protein-coding
Description adrenergic, beta, receptor kinase 1
Other Description G-protein coupled receptor kinase 2|beta-ARK-1|beta-adrenergic receptor kinase 1
Swissprots P25098 B0ZBE1 Q6GTT3 Q13837
Accessions AAB60689 ACA05909 EAW74581 EAW74582 P25098 AB209588 BAD92825 AI888604 AK131506 BC037963 AAH37963 BC090863 AAH90863 DN989901 DQ891148 ABM82074 DQ894328 ABM85254 M80776 AAA58391 X61157 CAA43470 XM_011544773 XP_011543075 XR_949796 NM_001619 NP_001610
Function Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner. {ECO:0000269|PubMed:19306925}.
Subcellular Location Cytoplasm {ECO:0000250|UniProtKB:P21146}. Cell membrane {ECO:0000250|UniProtKB:P21146}.
Tissue Specificity Expressed in peripheral blood leukocytes. {ECO:0000269|PubMed:10085131}.
Top Pathways Endocytosis, Chemokine signaling pathway, Glutamatergic synapse, Morphine addiction

GRK2 Antibody - 3982 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from Ramos, THP1, U-937, and COS cells, using GRK2 Antibody.

GRK 2 (C-15) - sc-562 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
GRK 2 (C-15): sc-562. Western blot analysis of GRK 2 expression in Ramos (A) and HL-60 (B) whole cell lysates.

GRK 2 (C-20) - sc-18409 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
GRK 2 (C-20): sc-18409. Western blot analysis of GRK 2 expression in HL-60 whole cell lysate.

GRK 2 (C-9) - sc-13143 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GRK 2 (C-9): sc-13143. Western blot analysis of GRK 2 expression in HL-60 (A), PC-3 (B), RAW 264.7 (C) and NAMALWA (D) whole cell lysates.

GRK 2 (E-7) - sc-166283 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GRK 2 (E-7): sc-166283. Western blot analysis of GRK 2 expression in non-transfected: sc-117752 (A) and human GRK 2 transfected: sc-111619 (B) 293T whole cell lysates.

GRK 2 (F-9) - sc-166284 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GRK 2 (F-9): sc-166284. Western blot analysis of GRK 2 expression in non-transfected 293T: sc-117752 (A), human GRK 2 transfected 293T: sc-111619 (B) and Ramos (C) whole cell lysates.

GRK 2 (G-12) - sc-271359 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GRK 2 (G-12): sc-271359. Western blot analysis of GRK 2 expression in non-transfected: sc-117752 (A) and human GRK 2 transfected: sc-111619 (B) 293T whole cell lysates.

GRK 2/3 (H-222) - sc-8329 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
GRK 2/3 (H-222): sc-8329. Immunofluorescence staining of methanol-fixed Ramos cells showing cytoplasmic and membrane staining.

Anti-GRK2 (phospho S29) antibody - ab58520 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
This image shows human breast carcinoma tissue stained with ab58520 at a 1/50 dilution. Right hand image: tissue treated with the immunising phosphopeptide. Left hand image; without the immunising phosphopeptide.

Anti-GRK2 (phospho S685) antibody - ab138438 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-GRK2 (phospho S685) antibody (ab138438) at 1/500 dilution + HT29 cells treated with 0.01 U/ml insulin for 15 min

Anti-GRK2 antibody - ab81578 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-GRK2 antibody (ab81578) at 1 µg/mlLane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 2 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell LysateLane 3 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell LysateLane 4 : Raji (Human Burkitt's lymphoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-GRK2 antibody - ab47999 from Abcam

Prices $376.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-GRK2 antibody (ab47999) at 0.3 µg/ml + Daudi cell lysate in RIPA buffer at 35 µg/ml

Anti-GRK2 antibody - ab153712 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-GRK2 antibody (ab153712) at 1/1000 dilution + Raji whole cell lysate at 30 µg

Anti-GRK2 antibody - ab64858 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-GRK2 antibody (ab64858) at 1/500 dilutionLane 1 : Extracts from HuvEc cellsLane 2 : Extracts from HuvEc cells with immunising peptide

Anti-GRK2 antibody - ab58521 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-GRK2 antibody [3F8] - ab113643 from Abcam

Prices $387.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3F8
Overlay histogram showing HeLa cells stained with ab113643 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab113643, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-GRK2 antibody [Y137] - ab32558 from Abcam

Prices $400.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone Y137
Anti-GRK2 antibody [Y137] (ab32558) at 1/500 dilution + U937 cell lysate

Anti-GRK2 antibody - C-terminal - ab137666 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-GRK2 antibody - C-terminal (ab137666) at 1/1000 dilution + NIH3T3 whole cell lysate at 30 µg

Anti-ADRBK1/2 Antibody (C-Terminus) - LS-C15619 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-GRK2 Antibody (phospho-Ser670) - LS-C11816 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-ADRBK1 / GRK2 Antibody - LS-C11814 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-ADRBK1 / GRK2 Antibody (aa468-689) - LS-C63769 from LifeSpan Bioscience

Prices $465.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-ADRBK1 / GRK2 Antibody (Asp635) - LS-C191955 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-ADRBK1 / GRK2 Antibody (aa14-63) - LS-C117651 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded human breast carcinoma tissue, using GRK2 (Ab-29) Antibody. The picture on the right is treated with the synthesized peptide.

Anti-ADRBK1 / GRK2 Antibody (aa35-84) - LS-C116435 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ADRBK1 / GRK2 antibody LS-C116435 Western blot of HCT15 cell lysate.
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