All lanes : Anti-GRK6 antibody (ab64915) at 1/500 dilutionLane 1 : Extracts from Jurkat cellsLane 2 : Extracts from COLO205 cellsLane 3 : Extracts from HUVEC cellsLane 4 : Extracts from HUVEC cells with immunising peptide at 10 µgLysates/proteins at 30 µg per lane.
ab64915, at a 1/500 dilution, staining Human GRK6 in HeLa cells, by Immunofluorescence, with (+) or without (-) immunising peptide.
IHC image of ab64915 staining in human normal heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab64915, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.