Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of colorectal cancer labeling GRP78 with ab181499 at 1/100. Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).
Immunofluorescent anaysis of HeLa cells (4% Paraformaldehyde-fixed) labeling GRP78 with ab181499 at 1/50 dilution.
All lanes : Anti-GRP78 BiP antibody [9E4-2A7-H6] - C-terminal (ab181499) at 1/1000 dilutionLane 1 : LnCap lysateLane 2 : C6 lysateLane 3 : MDA-MB-468 lysateLane 4 : HeLa lysate