Anti-GTF2IRD1 antibody [GTF5I102] (ab51524) at 1/50 dilution + Whole cell lysate from HeLa cells transfected with GTF2IRD1 expression construct at 50 µgSecondaryMouse IgG antibody at 1/2500 dilutiondeveloped using the ECL technique
ab51524 used at 5µg/500µg RIPA lysate of HeLa cells transfected with GTF2IRD1. Western blot was labelled with ab51524 at 1/50 dilution followed by Mouse IgG antibody at 1/2500 dilution. Developed using the ECL technique.
HeLa cells stained with ab51524 at 1/10 dilution followed by Alexa Fluor 488 Goat Anti Mouse IgG at 1/200 dilution. Nuclear stain: Hoescht 33342