Anti-HAUSP / USP7 antibody
| Name | Anti-HAUSP / USP7 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab4080 |
| Prices | $394.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ELISA WB ICC/IF ICC/IF IP IHC-P |
| Species Reactivities | Human |
| Antigen | Synthetic peptide: TDDPPRITQNPVC , corresponding to amino acids 30-41 of Human HAUSP |
| Description | Rabbit Polyclonal |
| Gene | USP7 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab4080 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4080, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Supervillin-mediated suppression of p53 protein enhances cell survival. - Supervillin-mediated suppression of p53 protein enhances cell survival.
Fang Z, Luna EJ. J Biol Chem. 2013 Mar 15;288(11):7918-29.
Deubiquitylase HAUSP stabilizes REST and promotes maintenance of neural - Deubiquitylase HAUSP stabilizes REST and promotes maintenance of neural
Huang Z, Wu Q, Guryanova OA, Cheng L, Shou W, Rich JN, Bao S. Nat Cell Biol. 2011 Feb;13(2):142-52.
Ubiquitin-specific proteases 7 and 11 modulate Polycomb regulation of the INK4a - Ubiquitin-specific proteases 7 and 11 modulate Polycomb regulation of the INK4a
Maertens GN, El Messaoudi-Aubert S, Elderkin S, Hiom K, Peters G. EMBO J. 2010 Aug 4;29(15):2553-65.
A SILAC-based DNA protein interaction screen that identifies candidate binding - A SILAC-based DNA protein interaction screen that identifies candidate binding
Mittler G, Butter F, Mann M. Genome Res. 2009 Feb;19(2):284-93.
The HAUSP gene plays an important role in non-small cell lung carcinogenesis - The HAUSP gene plays an important role in non-small cell lung carcinogenesis
Masuya D, Huang C, Liu D, Nakashima T, Yokomise H, Ueno M, Nakashima N, Sumitomo S. J Pathol. 2006 Apr;208(5):724-32.