All lanes : Anti-HAUSP / USP7 antibody (ab101648) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear LysateLane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell LysateLane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 6 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell LysateLane 7 : PC3 (Human prostate cancer cell line) Whole Cell Lysate (ab3954)Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab101648 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab101648 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-HAUSP / USP7 antibody (ab101648) at 1 µg/mlLane 1 : Testis (Mouse) Tissue Lysate Lane 2 : Testis (Rat) Tissue Lysate - normal tissue (ab29388)Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.