Anti-Hck antibody - N-terminal (ab175886) at 1/1000 dilution + A549 cell line lysate at 35 µg
Immunohistochemical analysis of Formalin-fixed, Paraffin-embedded Human spleen tissue labeling Hck with ab175886 at 1/10 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.
Flow Cytometric analysis of CEM cells labeling Hck with ab175886 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.