HCK

Gene Symbol HCK
Entrez Gene 3055
Alt Symbol JTK9, p59Hck, p61Hck
Species Human
Gene Type protein-coding
Description HCK proto-oncogene, Src family tyrosine kinase
Other Description hematopoietic cell kinase|hemopoietic cell kinase|p59-HCK/p60-HCK|tyrosine-protein kinase HCK
Swissprots A8K1I1 Q9NUA4 Q96CC0 Q29RX1 P08631 Q9UMJ5 Q9H5Y5 Q5T7K1 E1P5M2 Q2VPE2 B4DQB6 Q5T7K2 Q504R5
Accessions CAA41565 EAW76392 EAW76393 EAW76394 P08631 AK026432 BAB15482 AK225819 AK289896 BAF82585 AK290928 BAF83617 AK298726 BAG60878 AK314335 AW139272 BC014435 AAH14435 BC073748 AAH73748 BC094847 AAH94847 BC108930 AAI08931 BC108931 AAI08932 BC113854 AAI13855 BC114463 AAI14464 DA465978 M16592 AAA52644 NM_001172129 NP_001165600 NM_001172130 NP_001165601 NM_001172131 NP_001165602 NM_001172132 NP_001165603 NM_001172133 NP_001165604 NM_002110 NP_002101
Function Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation
Subcellular Location Cytoplasmic vesicle, secretory vesicle. Cytoplasm, cytosol.
Tissue Specificity Detected in monocytes and neutrophils (at protein level). Expressed predominantly in cells of the myeloid and B-lymphoid lineages. Highly expressed in granulocytes. Detected in tonsil. {ECO:0000269|PubMed:3453117, ECO:0000269|PubMed:8064233, ECO:0000269|PubMed:8995234}.
Top Pathways Fc gamma R-mediated phagocytosis, Chemokine signaling pathway

Hck (E1I7F) Rabbit mAb - 14643 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone E1I7F
Western blot analysis of extracts from GDM-1, THP-1, and HL-60 cell lines using Hck (E1I7F) Rabbit mAb.

Hck Antibody - 4352 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

Hck (G-4) - sc-166463 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Hck (G-4): sc-166463. Western blot analysis of Hck expression in non-transfected: sc-117752 (A) and mouse Hck transfected: sc-126948 (B) 293T whole cell lysates.

Hck (E-1) - sc-166402 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Hck (E-1): sc-166402. Western blot analysis of Hck expression in HL-60 whole cell lysate.

Hck (3D12E10) - sc-101428 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Hck (3D12E10): sc-101428. Western blot analysis of Hck expression in U-937 (A) and HL-60 (B) whole cell lysates.

Hck (H-85) - sc-11401 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Hck (H-85): sc-11401. Western blot analysis of Hck expression in non-transfected 293T: sc-117752 (A), mouse Hck transfected 293T: sc-126948 (B) and HL-60 (C) whole cell lysates.

Hck (N-30) - sc-72 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Hck (N-30): sc-72. Western blot analysis of Hck expression in U-937 (A) and HL-60 (B) whole cell lysates.

Hck (18) - sc-136289 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
Hck (18): sc-136289. Western blot analysis of Hck expression in MDCK whole cell lysate.

p-Hck (Tyr 411)-R - sc-12958-R from Santa Cruz Biotechnology

Prices $61.00
Sizes 100 µg/0.5 ml
Host Rabbit
Clonality Polyclonal

Anti-Hck (phospho Y410) antibody - ab61055 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab61055 staining Hck (phospho Y410) in Mouse lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% formalin and blocked with 10% BSA for 1 hour; antigen retrieval was by heat mediation in trypsin. Samples were incubated with primary antibody (1/100) for 24 hours. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.See Abreview

Anti-Hck (phospho Y521) antibody - ab192578 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Hck (phospho Y521) antibody (ab192578) at 1/500 dilutionLane 1 : Jurkat cell lysateLane 2 : Jurkat cell lysate with Immunizing peptide

Anti-Hck antibody - ab32860 from Abcam

Prices $372.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-Hck antibody - ab63352 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence analysis of Hck expression in HepG2 cells, using ab63352 at 1/500. Left: untreated cells. Right: cells treated with immunising peptide.

Anti-Hck antibody - ab124245 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-Hck antibody (ab124245) at 1/100 dilution + CEM cell line lysates at 35 µg

Anti-Hck antibody - ab171905 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-Hck antibody (ab171905) at 1 µg/mlLane 1 : Hck transfected 293T cell line lysateLane 2 : Non-transfected 293T cell line lysateLysates/proteins at 15 µl per lane.developed using the ECL technique

Anti-Hck antibody [3D12E10] - ab63586 from Abcam

Host Mouse
Clonality Monoclonal
Clone 3D12E10
All lanes : Anti-Hck antibody [3D12E10] (ab63586) at 1/500 dilutionLane 1 : Immunising protein (recombinant fragment)Lane 2 : Cos 7 cell lysate transfected with full length Hck-GFP

Anti-Hck antibody [EP2415Y] - ab75839 from Abcam

Prices $99.00
Sizes 10 µl
Host Rabbit
Clonality Monoclonal
Clone EP2415Y
Anti-Hck antibody [EP2415Y] (ab75839) at 1/200000 dilution + TF-1 cell lysate at 10 µgSecondarygoat anti-rabbit HRP at 1/2000 dilution

Anti-Hck antibody - N-terminal - ab175886 from Abcam

Prices $384.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-Hck antibody - N-terminal (ab175886) at 1/1000 dilution + A549 cell line lysate at 35 µg

Anti-HCK Antibody - LS-C331879 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of HL-60 cell lines, using HCK antibody.

Anti-HCK Antibody (aa131-156) - LS-C166814 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
HCK Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human spleen tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Anti-HCK Antibody (aa239-272) - LS-C344307 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot of lysate from mouse thymus tissue lysate with Mouse Hck Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35 ug.

Anti-HCK Antibody (aa29-43) - LS-C7332 from LifeSpan Bioscience

Prices $465.00
Sizes 200 µg
Host Rabbit
Clonality Polyclonal

Anti-HCK Antibody (aa22-80) - LS-C36599 from LifeSpan Bioscience

Prices $465.00
Sizes 100 µg
Host Rat
Clonality Monoclonal

Anti-HCK Antibody (aa1-80) - LS-C129548 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-HCK Antibody (aa381-430) - LS-C117904 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence of HepG2 cells, using HCK (Ab-410) Antibody. The picture on the right is treated with the synthesized peptide.
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