Anti-HCN4 antibody [S114-10]
| Name | Anti-HCN4 antibody [S114-10] |
|---|---|
| Supplier | Abcam |
| Catalog | ab85023 |
| Prices | $379.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | S114-10 |
| Applications | IHC-F WB ICC/IF ICC/IF IHC-P FC |
| Species Reactivities | Mouse, Rat, Human, Rainbow trout |
| Antigen | Fusion protein: PRGGLSPPGH SPGPPRTFPS APPRASGSHG SLLLPPASSP PPPQVPQRRG TPPLTPGRLT QDLKLISASQ PALPQDGAQT LRRASPHSSG, corresponding to cytoplasmic C terminal amino acids 1019-1108 of Rat HCN4 (Q9JKA7) |
| Description | Mouse Monoclonal |
| Gene | HCN4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab85023 staining HCN4 in human hippocampus tissue section by IHC-P. Bouin's fixed and paraffin-embedded tissue sections were used.
![Overlay histogram showing SH-SY5Y cells stained with ab85023 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab85023, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/29203_HCN4-Primary-antibodies-ab85023-4.jpg)
Overlay histogram showing SH-SY5Y cells stained with ab85023 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab85023, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
In vitro developed spontaneously contracting cardiomyocytes from rainbow trout as - In vitro developed spontaneously contracting cardiomyocytes from rainbow trout as
Grunow B, Wenzel J, Terlau H, Langner S, Gebert M, Kruse C. Cell Physiol Biochem. 2011;27(1):1-12.
Redox stimulation of cardiomyogenesis versus inhibition of vasculogenesis upon - Redox stimulation of cardiomyogenesis versus inhibition of vasculogenesis upon
Milosevic N, Bekhite MM, Sharifpanah F, Ruhe C, Wartenberg M, Sauer H. Antioxid Redox Signal. 2010 Dec 15;13(12):1813-27.