HCN4

Gene Symbol HCN4
Entrez Gene 10021
Alt Symbol SSS2
Species Human
Gene Type protein-coding
Description hyperpolarization activated cyclic nucleotide gated potassium channel 4
Other Description hyperpolarization activated cyclic nucleotide-gated cation channel 4|hyperpolarization activated cyclic nucleotide-gated potassium channel 4|potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4
Swissprots Q9UMQ7 Q9Y3Q4
Accessions CCF23300 EAW77925 Q9Y3Q4 AJ132429 CAB42604 AJ238850 CAB52754 BC156298 AAI56299 BC172471 AAI72471 BM979827 BP291686 DQ854814 XM_011521148 XP_011519450 NM_005477 NP_005468
Function Hyperpolarization-activated ion channel with very slow activation and inactivation exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) that regulate the rhythm of heart beat. May contribute to the native pacemaker currents in neurons (Ih). May mediate responses to sour stimuli. {ECO:0000269|PubMed:10228147, ECO:0000269|PubMed:10430953, ECO:0000269|PubMed:16407510, ECO:0000269|PubMed:19165230, ECO:0000269|PubMed:20829353}.
Subcellular Location Cell membrane {ECO:0000269|PubMed:10228147, ECO:0000269|PubMed:10430953, ECO:0000269|PubMed:16407510}; Multi- pass membrane protein {ECO:0000269|PubMed:10228147, ECO:0000269|PubMed:10430953, ECO:0000269|PubMed:16407510}.
Tissue Specificity Highly expressed in thalamus, testis and in heart, both in ventricle and atrium. Detected at much lower levels in amygdala, substantia nigra, cerebellum and hippocampus. {ECO:0000269|PubMed:10228147, ECO:0000269|PubMed:10430953}.
Top Pathways cAMP signaling pathway

HCN4 (C-16) - sc-19714 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
HCN4 (C-16): sc-19714. Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.

HCN4 (H-300) - sc-28750 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

HCN4 (SHG 1E5) - sc-58622 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rat
Clonality Monoclonal
HCN4 (SHG 1E5): sc-58622. Western blot analysis of HCN4 expression in non-transfected: sc-117752 (A) and human HCN4 transfected: sc-173585 (B) 293T whole cell lysates.

Anti-HCN2 + HCN4 antibody - ab74729 from Abcam

Host Rabbit
Clonality Polyclonal

Anti-HCN4 antibody - ab69054 from Abcam

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-HCN4 antibody (ab69054) at 1 µg/mlLane 1 : Heart (Human) Whole Cell Lysate - fetal normal tissueLane 2 : Heart (Rat) Tissue LysateLane 3 : Heart (Mouse) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-HCN4 antibody - ab66501 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab66501 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66501, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

Anti-HCN4 antibody - ab184187 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-HCN4 antibody (ab184187) at 1 µg/mlLane 1 : Brain (Mouse) Tissue LysateLane 2 : Brain (Rat) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-HCN4 antibody - ab65702 from Abcam

Host Rabbit
Clonality Polyclonal

Anti-HCN4 antibody [S114-10] - ab85023 from Abcam

Prices $379.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone S114-10
ab85023 staining HCN4 in human hippocampus tissue section by IHC-P. Bouin's fixed and paraffin-embedded tissue sections were used.

Anti-HCN4 antibody [SHG 1E5] - ab32675 from Abcam

Prices $390.00
Sizes 100 µl
Host Rat
Clonality Monoclonal
Clone SHG 1E5
Overlay histogram showing PC12 cells stained with ab32675 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32675, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG1 [RTK2071] (ab18412, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in PC12 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.

Anti-HCN4 Antibody (C-Terminus) - LS-C7346 from LifeSpan Bioscience

Prices $385.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-HCN4 Antibody (C-Terminus) - LS-C7345 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-HCN4 Antibody (C-Terminus) - LS-C123683 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-HCN4 Antibody (aa1019-1198, clone S114-10, Atto 488) - LS-C227695 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone S114-10

Anti-HCN4 Antibody (clone S114-10) - LS-C152675 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone S114-10

Anti-HCN4 Antibody (aa1019-1198, clone S114-10, Atto 565) - LS-C227699 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone S114-10

Anti-HCN4 Antibody (C-Terminus) - LS-C144781 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-HCN4 Antibody (aa1019-1198, clone S114-10, PerCP) - LS-C227701 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone S114-10

Anti-HCN4 Antibody (aa1019-1198, clone S114-10, Atto 390) - LS-C227696 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone S114-10

Anti-HCN4 Antibody (aa1019-1198, clone S114-10, RPE) - LS-C227702 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone S114-10

Anti-HCN4 Antibody (aa1019-1198, clone S114-10, Atto 594) - LS-C227700 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone S114-10

Anti-HCN4 Antibody (aa1019-1198, clone S114-10, SA) - LS-C227703 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone S114-10

Anti-HCN4 Antibody (aa1019-1198, clone S114-10, Atto 633) - LS-C229865 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone S114-10

Anti-HCN4 Antibody (aa1019-1198, clone S114-10, Biotin) - LS-C229871 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone S114-10

Anti-HCN4 Antibody (aa1019-1198, clone S114-10, Atto 700) - LS-C229868 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone S114-10
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