Anti-HCN4 antibody [SHG 1E5]
| Name | Anti-HCN4 antibody [SHG 1E5] |
|---|---|
| Supplier | Abcam |
| Catalog | ab32675 |
| Prices | $390.00 |
| Sizes | 100 µl |
| Host | Rat |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | SHG 1E5 |
| Applications | WB ICC/IF ICC/IF IHC-F FC IHC-P |
| Species Reactivities | Mouse, Rat, Human, Chimpanzee |
| Antigen | Synthetic peptide: SHGSLLLPPA SSPPPPQVPQ RRGTPPLTPG RLTQDLKL, corresponding to amino acids 1048-1085 of Human HCN4 |
| Description | Rat Monoclonal |
| Gene | HCN4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing PC12 cells stained with ab32675 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32675, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG1 [RTK2071] (ab18412, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in PC12 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/29204_HCN4-Primary-antibodies-ab32675-2.jpg)
Overlay histogram showing PC12 cells stained with ab32675 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32675, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG1 [RTK2071] (ab18412, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in PC12 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.
Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human tonsil tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rat monoclonal antibody recognizing HCN4 ab32675 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human heart tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rat monoclonal antibody recognizing HCN4 ab32675 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Product References
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Oxidized CaMKII causes cardiac sinus node dysfunction in mice. - Oxidized CaMKII causes cardiac sinus node dysfunction in mice.
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Differentiation induction of mouse embryonic stem cells into sinus node-like - Differentiation induction of mouse embryonic stem cells into sinus node-like
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DSCAM and DSCAML1 function in self-avoidance in multiple cell types in the - DSCAM and DSCAML1 function in self-avoidance in multiple cell types in the
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The tight junction protein CAR regulates cardiac conduction and cell-cell - The tight junction protein CAR regulates cardiac conduction and cell-cell
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HCN4-like immunoreactivity in rat retinal ganglion cells. - HCN4-like immunoreactivity in rat retinal ganglion cells.
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Coxsackievirus and adenovirus receptor (CAR) mediates atrioventricular-node - Coxsackievirus and adenovirus receptor (CAR) mediates atrioventricular-node
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