Anti-HCP1 antibody
| Name | Anti-HCP1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab25134 |
| Prices | $380.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ELISA ICC/IF ICC/IF WB |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Mouse HCP1 |
| Description | Rabbit Polyclonal |
| Gene | SLC46A1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-HCP1 antibody (ab25134) at 1 µg/mlLane 1 : Liver (Rat) Tissue Lysate Lane 2 : Spleen (Rat) Tissue LysateLane 3 : Heart (Rat) Tissue LysateLane 4 : Kidney (Mouse) Tissue LysateLane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Lysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
All lanes : Anti-HCP1 antibody (ab25134) at 1 µg/mlLane 1 : Kidney (Mouse) Tissue LysateLane 2 : Kidney (Rat) Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab25134 stained NIH-3T3 cells. The cells were 4% paraformladhyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25134, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Image courtesy of Human Protein Atlasab25134 staining HCP1 in human stomach. Paraffin embedded human skin tissue was incubated with ab25134 (1/100 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab25134 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org.
Product References
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