All lanes : Anti-HDAC1 antibody [6F11] (ab114030) at 1/500 dilutionLane 1 : HEK293T cells were transfected with the pCMV6-ENTRY control cDNA for 48 hrs and lysedLane 2 : HEK293T cells were transfected with the pCMV6-ENTRY HDAC1 cDNA for 48 hrs and lysedLysates/proteins at 5 µg per lane.
Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HDAC1, using ab114030 at 1/100
HEK293T cells transfected with either RC201745 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ab114030 at 1/100 and then analyzed by flow cytometry