HDAC1

Gene Symbol HDAC1
Entrez Gene 3065
Alt Symbol GON-10, HD1, RPD3, RPD3L1
Species Human
Gene Type protein-coding
Description histone deacetylase 1
Other Description reduced potassium dependency, yeast homolog-like 1
Swissprots Q92534 Q13547
Accessions BAG70921 EAX07540 EAX07541 Q13547 AB451297 BAG70111 AB451430 BAG70244 AK225555 AK296308 BAH12310 AK299241 BAG61272 AK299792 BAG61671 AK313061 AY627042 AAT44863 BC000301 AAH00301 BX648055 CAI46269 CR541717 CAG46518 D50405 BAA08909 DQ891398 ABM82324 DQ894577 ABM85503 U50079 AAC50475 XM_011541309 XP_011539611 NM_004964 NP_004955
Function Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST- mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B. Deacetylates NR1D2 and abrogates the effect of KAT5-mediated relieving of NR1D2 transcription repression activity. Component of a RCOR/GFI/KDM1A
Subcellular Location Nucleus.
Tissue Specificity Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
Top Pathways Notch signaling pathway, MicroRNAs in cancer, Alcoholism, Epstein-Barr virus infection, Viral carcinogenesis

Histone Deacetylase 1 (HDAC1) Antibody - 2062 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of COS or HeLa cell lysates using Histone Deacetylase 1 (HDAC1) Antibody.

HDAC1 (10E2) Mouse mAb - 5356 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 10E2
Western blot analysis of extracts from HeLa, NIH/3T3, and H-4-II-E cells using HDAC1 (10E2) Mouse mAb.

HDAC1 (10E2) - sc-81598 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
HDAC1 (10E2): sc-81598. Western blot analysis of HDAC1 expression in NIH/3T3 (A), HeLa (B), K-562 (C), Jurkat (D), KNRK (E) and C32 (F) nuclear extracts and human recombinant HDAC1 fusion protein(G).

HDAC1 (C-19) - sc-6298 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
HDAC1 (C-19): sc-6298. Western blot analysis of HDAC1 expression in K-562 (A), Jurkat (B) and NIH/3T3 (C) nuclear extracts.

HDAC1 (H-11) - sc-8410 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ChIP analysis of <i>c</i>-<i>Myc</i> promoter occupancy in response to lithium stimulation in serum synchronized C2C12 cells. Antibodies tested include HDAC1 (H-15): sc-7872, HDAC1 (C-19): sc-6298 and HDAC1 (H-11): sc-8410 (A), β-catenin (H-120): sc-7199, β-catenin (C-18): sc-1496 and β-catenin (E-5): sc-7963 (B), E2F-4 (A-20): sc-1082, E2F-4 (D-3): sc-6851, E2F-4 (RK-13): sc-511 and E2F-4 (C-20): sc-866 (C), and p130 (C-20): sc-317, p130 (211.6): sc-9963 and p130 (H-125): sc-20678 (D). Data kindly provided by M.G. Rosenfeld and reproduced with permission from Proc. Natl. Acad. Sci. 100: 3245-3250. Copyright 2003 National Academy of Sciences, USA.

HDAC1 (H-51) - sc-7872 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
HDAC1 (H-51): sc-7872. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.

HDAC1 (N-19) - sc-6299 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
HDAC1 (N-19): sc-6299. Western blot analysis of HDAC1 expression in NIH/3T3 (A) and KNRK (B) nuclear extracts.

Anti-dHDAC1 (phospho S421 + S423) antibody - C-terminal - ab138384 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labelling dHDAC1 (S421/S423) with ab138384 at 1/50.

Anti-HDAC1 antibody - ab53091 from Abcam

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-HDAC1 antibody (ab53091) at 1/300 dilutionLane 1 : NIH/3T3 cell extractsLane 2 : NIH/3T3 cell extracts with blocking peptide

Anti-HDAC1 antibody - ab55094 from Abcam

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
HDAC1 antibody (ab55094) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human liver.

Anti-HDAC1 antibody - ab11966 from Abcam

Prices $378.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
HDAC1 detection by Western blot. The analysis of HDAC1 in 293 cell extract with anti-HDAC1 pAb. A protein band of approximate molecular weight of 60 kDa was detected.

Anti-HDAC1 antibody - ab46851 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-HDAC1 antibody (ab46851) at 2 µg/ml + 293 cell lysate

Anti-HDAC1 antibody [10E2] - ChIP Grade - ab46985 from Abcam

Prices $403.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 10E2
All lanes : Anti-HDAC1 antibody [10E2] - ChIP Grade (ab46985) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-HDAC1 antibody [5C3] - ab114023 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5C3
HEK293T cells transfected with either RC201745 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ab114023 at 1/100, and then analyzed by flow cytometry.

Anti-HDAC1 antibody [6F11] - ab114030 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 6F11
All lanes : Anti-HDAC1 antibody [6F11] (ab114030) at 1/500 dilutionLane 1 : HEK293T cells were transfected with the pCMV6-ENTRY control cDNA for 48 hrs and lysedLane 2 : HEK293T cells were transfected with the pCMV6-ENTRY HDAC1 cDNA for 48 hrs and lysedLysates/proteins at 5 µg per lane.

Anti-HDAC1 antibody [EPR460(2)] - ab109411 from Abcam

Prices $401.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR460(2)
ab109411 (1/400) staining HDAC1 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.See Abreview

Anti-HDAC1 antibody [EPR460(2)] (Alexa Fluor® 488) - ab192469 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR460(2)
ab192469 staining HDAC1 in HeLa cells. The cells were fixed with 100% methanal (5 min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Triton X-100 for 1hr. The cells were then incubated with ab192469 at a working dilution of 1 in 100 (shown in green) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1hr with Alexa Fluor® 594 Goat anti-mouse IgG (H&L - preadsorbed) (ab150120) at 2 μg/ml (shown in pseudo-color red). Nuclear DNA was labelled in blue with DAPI.This product gave a positive signal in 4% formaldehyde (10 min) fixed HeLa cells under the same testing conditions.Image was taken with a Confocal microscope (Leica-microsystems, TCS SP8).

Anti-HDAC1 antibody [EPR460(2)] (Alexa Fluor® 647) - ab192470 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR460(2)
ab192470 staining HDAC1 in HeLa cells. The cells were fixed with 100% methanol (5 min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Triton X-100 for 1hr. The cells were then incubated with ab192470 at a working dilution of 1 in 100 (shown in red) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1hr with AlexaFluor® 488 Goat anti-mouse IgG (H&L - preadsorbed) (ab150117) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.This product gave a positive signal in 4% formaldehyde (10 min) fixed HeLa cells tested under the same conditions.Image was taken with a Confocal microscope (Leica-microsystems, TCS SP8).

Anti-HDAC1 antibody [EPR460(2)] (HRP) - ab193096 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR460(2)
IHC image of HDAC1 staining in a section of formalin-fixed paraffin-embedded normal human pancreas*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30 mins. The section was incubated with ab193096 at a working dilution of 1 in 500 overnight at +4°C. The section was counterstained with haematoxylin and mounted with DPX.The inset negative control image is taken from an identical assay without primary antibody.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Anti-HDAC1 antibody [EPR5517(2)] - ab150399 from Abcam

Prices $388.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5517(2)
All lanes : Anti-HDAC1 antibody [EPR5517(2)] (ab150399) at 1/1000 dilutionLane 1 : K562 cell lysateLane 2 : Jurkat cell lysateLane 3 : MCF7 cell lysateLane 4 : HeLa cell lysateLysates/proteins at 10 µg per lane.

Anti-HDAC1 antibody [HDAC1-21] - ab12168 from Abcam

Prices $373.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone HDAC1-21

Anti-HDAC1 antibody, prediluted - ab15316 from Abcam

Prices $382.00
Sizes 7 ml
Host Rabbit
Clonality Polyclonal
ab15316 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab15316 at 1/50 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-HDAC1 antibody - ChIP Grade - ab7028 from Abcam

Prices $403.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
HeLa cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone. Fixed cells were stained with 1:200 ab7028. The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.

Anti-HDAC1 Antibody (aa368-417) - LS-C292049 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-HDAC1 Antibody (N-Terminus) - LS-C286012 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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