Standard Curve for HDAC3 (Analyte: HDAC3 protein (Tagged) (ab82071)); dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal [7G6C5] to HDAC3 (ab76359) at 5 ug/ml and Detector Antibody Rabbit polyclonal to HDAC3 (ab63353) at 0.5 ug/ml.
All lanes : Anti-HDAC3 antibody (ab63353) at 1/500 dilutionLane 1 : extracts from NIH/3T3 cells, without immunizing peptideLane 2 : extracts from NIH/3T3 cells, with immunizing peptide
Immunohistochemical analysis of HDAC3 expression in paraffin-embedded human skin tissue, using ab63353 at 1/50 dilution. Right: sample treated with immunising peptide. Left: untreated sample.
Immunofluorescence analysis of HDAC3 expression in HeLa cells, using ab63353 at 1/500 dilution. Left: Cells treated with TNF-a (20nM, 15mins). Right: Untreated cells.