Anti-HDAC6 antibody
| Name | Anti-HDAC6 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab12173 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IP |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic peptide: KNIAHQNKFGEDMPH conjugated to KLH, corresponding to amino acids 1199-1213 of Human HDAC6 |
| Description | Rabbit Polyclonal |
| Gene | HDAC6 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-HDAC6 antibody (ab12173) at 1/500 dilutionLane 1 : Whole cell lysate prepared from Human HepG2 cell line.Lane 2 : Whole cell lysate prepared from Human HepG2 cell line.Lane 3 : Whole cell lysate prepared from Human HepG2 cell line.Lysates/proteins at 20 µg per lane.SecondaryHRP conjugated goat anti-rabbit IgG at 1/2000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 150 kDa (why is the actual band size different from the predicted?)Exposure time : 1 minuteThis image is courtesy of an Abreview submitted by Cheng SongSee Abreview
ab12173 staining mouse NIH3T3 cells by ICC/IF. Cells were formaldehyde fixed and permeabilized in 0.2% Triton X-100 prior to blocking in 10% serum for 1 hour at 25°C . The primary antibody was diluted 1/50 and incubated with sample for 1 hour at 25°C . A Alexa Fluor® 488 goat polyclonal to rabbit IgG was used as the secondary, diluted 1/200.See Abreview
Product References
Monoaminergic and neuropeptidergic neurons have distinct expression profiles of - Monoaminergic and neuropeptidergic neurons have distinct expression profiles of
Takase K, Oda S, Kuroda M, Funato H. PLoS One. 2013;8(3):e58473.
Protein aggregates are recruited to aggresome by histone deacetylase 6 via - Protein aggregates are recruited to aggresome by histone deacetylase 6 via
Ouyang H, Ali YO, Ravichandran M, Dong A, Qiu W, MacKenzie F, Dhe-Paganon S, Arrowsmith CH, Zhai RG. J Biol Chem. 2012 Jan 20;287(4):2317-27.
Sepsis and glucocorticoids upregulate p300 and downregulate HDAC6 expression and - Sepsis and glucocorticoids upregulate p300 and downregulate HDAC6 expression and
Alamdari N, Smith IJ, Aversa Z, Hasselgren PO. Am J Physiol Regul Integr Comp Physiol. 2010 Aug;299(2):R509-20. doi: