HDAC6

Gene Symbol HDAC6
Entrez Gene 10013
Alt Symbol CPBHM, HD6, JM21, PPP1R90
Species Human
Gene Type protein-coding
Description histone deacetylase 6
Other Description protein phosphatase 1, regulatory subunit 90
Swissprots Q96CY0 Q6NT75 O94975 Q9UBN7 Q7L3E5
Accessions CAF04396 EAW50744 EAW50745 EAW50746 EAW50747 EAW50748 Q9UBN7 AB020708 BAA74924 AF132609 AAD29048 AF174499 AAF36540 AJ011972 CAA09893 AK001543 BAG50935 AK024083 BAG51263 AK095124 BAG52988 AK122954 BAG53817 AK127118 AK131286 BAG54760 AK295884 BAG58680 AK302926 BAG64088 AK303012 BAG64143 AK309058 AL137696 CAB70878 BC005872 AAH05872 BC011498 AAH11498 BC013219 BC013737 AAH13737 BC069243 AAH69243 BT006649 AAP35295 JF432329 ADZ15546 XM_005272564 XP_005272621 XM_005272565 XP_005272622 XM_005272566 XP_005272623 XM_005272568 XP_005272625 XM_006724525 XP_006724588 XR_246712 XR_949014 NM_006044 NP_006035
Function Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. {ECO:0000250}.
Subcellular Location Nucleus. Cytoplasm. Perikaryon {ECO:0000250}. Cell projection, dendrite {ECO:0000250}. Cell projection, axon {ECO:0000250}. Note=It is mainly cytoplasmic, where it is associated with microtubules.
Top Pathways Viral carcinogenesis, Alcoholism

HDAC6 (D2E5) Rabbit mAb - 7558 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D2E5
Western blot analysis of extracts from various cell lines using HDAC6 (D2E5) Rabbit mAb.

HDAC6 (D21B10) Rabbit mAb - 7612 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D21B10
Western blot analysis of extracts from HeLa and Jurkat cells using HDAC6 (D21B10) Rabbit mAb.

Histone Deacetylase 6 (HDAC6) Antibody - 2162 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

HDAC6 (D-11) - sc-28386 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
HDAC6 (D-11): sc-28386. Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver cancer showing cytoplasmic staining of tumor cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

HDAC6 (H-300) - sc-11420 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
HDAC6 (H-300): sc-11420. Western blot analysis of HDAC6 expression in K-562 whole cell lysate.

HDAC6 (L-18) - sc-5258 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
HDAC6 (L-18): sc-5258. Western blot analysis of HDAC6 expression in K-562 nuclear extract.

HDAC6 (S-14) - sc-5255 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
HDAC6 (S-14): sc-5255. Western blot analysis of HDAC6 expression in K-562 nuclear extract.

Anti-HDAC6 (phospho S22) antibody - ab61058 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry analysis of paraffin-embedded human testis tissue using HDAC6 (phospho S22) antibody (ab61058) at 1/50 - 1/100 dilution, in the presence (right panel) and absence (left panel) of phosphopeptide.

Anti-HDAC6 antibody - ab1440 from Abcam

Prices $392.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-HDAC6 antibody (ab1440) + Jurkat cell lysate

Anti-HDAC6 antibody - ab12173 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-HDAC6 antibody (ab12173) at 1/500 dilutionLane 1 : Whole cell lysate prepared from Human HepG2 cell line.Lane 2 : Whole cell lysate prepared from Human HepG2 cell line.Lane 3 : Whole cell lysate prepared from Human HepG2 cell line.Lysates/proteins at 20 µg per lane.SecondaryHRP conjugated goat anti-rabbit IgG at 1/2000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 150 kDa (why is the actual band size different from the predicted?)Exposure time : 1 minuteThis image is courtesy of an Abreview submitted by Cheng SongSee Abreview

Anti-HDAC6 antibody - ab56926 from Abcam

Prices $390.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Overlay histogram showing HeLa cells stained with ab56926 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56926, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was an anti-mouse Alexa Fluor® 488 (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-HDAC6 antibody - ab117516 from Abcam

Prices $384.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab117516, at 5 µg/ml, staining HDAC6 in Formalin-fixed, Paraffin-embedded Human intestine tissue (ganglion cells) by Immunohistochemistry.

Anti-HDAC6 antibody - ab99336 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-HDAC6 antibody (ab99336) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgdeveloped using the ECL technique

Anti-HDAC6 antibody - ab53099 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC image of HDAC6  staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab53099, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-HDAC6 antibody - ab82557 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-HDAC6 antibody (ab82557) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 4 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-HDAC6 antibody - ab11970 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
HDAC6 detection by Western blot. The analysis of HDAC6 in NIH/3T3 cell extract with anti-HDAC6 pAb. A protein band of approximate molecular weight of 134 kDa was

Anti-HDAC6 antibody - ab185938 from Abcam

Prices $376.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-HDAC6 antibody (ab185938) at 3 µg/ml + HeLa nuclear cell lysate at 35 µg

Anti-HDAC6 antibody - ab61173 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
1/50-1/100 ab61173 staining HDAC6 in human testis tissue, in the absence or presence of the immunizing peptide.

Anti-HDAC6 antibody [1D10] - ab117900 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1D10
HEK293T cells transfected with eitherHDAC6-overexpressing plasmid (Red) or empty vector control plasmid (Blue) were immunostained with 1/100 ab117900, and then analyzed by flow cytometry.

Anti-HDAC6 antibody [EPR1698(2)] - ab133493 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1698(2)
All lanes : Anti-HDAC6 antibody [EPR1698(2)] (ab133493) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : Jurkat cell lysateLane 3 : K562 cell lysateLane 4 : COS-1 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-HDAC6 antibody [EPR1698(2)] (Alexa Fluor® 488) - ab202948 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1698(2)
ab202948 staining HDAC6 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202948 at 1/200 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-HDAC6 antibody [EPR1699(2)] - ab124700 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1699(2)
All lanes : Anti-HDAC6 antibody [EPR1699(2)] (ab124700) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : Jurkat cell lysateLane 3 : K562 cell lysateLane 4 : COS1 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution

Anti-HDAC6 antibody [EPR6159] - ab133541 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6159
All lanes : Anti-HDAC6 antibody [EPR6159] (ab133541) at 1/1000 dilutionLane 1 : 293T cell lysateLane 2 : HUVEC cell lysateLane 3 : HeLa cell lysateLane 4 : MCF7 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-HDAC6 antibody [EPR6160] - ab133539 from Abcam

Prices $381.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6160
All lanes : Anti-HDAC6 antibody [EPR6160] (ab133539) at 1/1000 dilutionLane 1 : 293T cell lysateLane 2 : HepG2 cell lysateLane 3 : K562 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-HDAC6 antibody - C-terminal - ab196351 from Abcam

Prices $376.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-HDAC6 antibody - C-terminal (ab196351) at 1 µg/ml + HeLa nuclear lysate at 35 µg
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