Anti-HDAC6 antibody
| Name | Anti-HDAC6 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab56926 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | WB ICC/IF ICC/IF FC |
| Species Reactivities | Human |
| Antigen | Recombinant fragment: DVTQPCGDCG TIQENWVCLS CYQVYCGRYI NGHMLQHHGN SGHPLVLSYI DLSAWCYYCQ AYVHHQALLD VKNIAHQNKF GEDMPHPH, corresponding to amino acids 1128-1216 of Human HDAC6 Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | HDAC6 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HeLa cells stained with ab56926 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56926, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was an anti-mouse Alexa Fluor® 488 (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/29460_ab56926-3-ab56926FC.jpg)
Overlay histogram showing HeLa cells stained with ab56926 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56926, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was an anti-mouse Alexa Fluor® 488 (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
ICC/IF image of ab56926 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56926, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Histone deacetylase inhibitors sensitize lung cancer cells to hyperthermia: - Histone deacetylase inhibitors sensitize lung cancer cells to hyperthermia:
Hassan MK, Watari H, Salah-Eldin AE, Sultan AS, Mohamed Z, Fujioka Y, Ohba Y, Sakuragi N. PLoS One. 2014 Apr 11;9(4):e94213.
Ankyrin repeat and suppressor of cytokine signaling box containing protein-10 is - Ankyrin repeat and suppressor of cytokine signaling box containing protein-10 is
Keller KE, Yang YF, Sun YY, Sykes R, Acott TS, Wirtz MK. Mol Vis. 2013 Jul 25;19:1639-55. Print 2013.
Histone deacetylase 6 (HDAC6) plays a crucial role in p38MAPK-dependent induction - Histone deacetylase 6 (HDAC6) plays a crucial role in p38MAPK-dependent induction
Kastle M, Woschee E, Grune T. Free Radic Biol Med. 2012 Dec 1;53(11):2092-101. doi:
Proteasome inhibition drives HDAC6-dependent recruitment of tau to aggresomes. - Proteasome inhibition drives HDAC6-dependent recruitment of tau to aggresomes.
Guthrie CR, Kraemer BC. J Mol Neurosci. 2011 Sep;45(1):32-41.