HEK293T cells transfected with eitherHDAC6-overexpressing plasmid (Red) or empty vector control plasmid (Blue) were immunostained with 1/100 ab117900, and then analyzed by flow cytometry.
Immunofluorescent staining of COS7 transiently transfected cells with HDAC6 cDNA, using 1/100 ab117900.
All lanes : Anti-HDAC6 antibody [1D10] (ab117900) at 1/500 dilutionLane 1 : mock-transfected HEK293T cell lysateLane 2 : HDAC6-transfected HEK293T cell lysateLysates/proteins at 5 µg per lane.
All lanes : Anti-HDAC6 antibody [1D10] (ab117900) at 1/500 dilutionLane 1 : HepG2 cell extractsLane 2 : HeLa cell extractsLane 3 : HT29 cell extractsLane 4 : A549 cell extractsLane 5 : COS7 cell extractsLane 6 : Jurkat cell extractsLane 7 : MDCK cell extractsLane 8 : PC12 cell extractsLane 9 : MCF7 cell extractsLysates/proteins at 35 µg per lane.