Anti-HDAC9 antibody
| Name | Anti-HDAC9 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab53102 |
| Prices | $379.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ELISA ICC/IF ICC/IF WB |
| Species Reactivities | Human |
| Antigen | Synthetic peptide derived from human HDAC9 |
| Description | Rabbit Polyclonal |
| Gene | HDAC9 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab53102 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab53102 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Dylight® 488 goat anti- rabbit (ab96899) IgG (H+L) preadsorbed, used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
All lanes : Anti-HDAC9 antibody (ab53102) at 1/300 dilutionLane 1 : HepG2 cell extractLane 2 : HepG2 cell extract with peptide