Anti-Heme Oxygenase 1 antibody [EP1391Y]

• Supplier: Abcam
• Catalog: ab52947
• Prices: $400.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP1391Y
• Applications: WB IP FC IHC-P
• Species Reactivities: Mouse, Guinea Pig, Human
• Antigen: A synthetic peptide corresponding to residues near the C-terminus of human Heme Oxygenase 1
• Description: Rabbit Monoclonal
• Gene: HMOX1
• Conjugate: Unconjugated

Product images

Overlay histogram showing HEK293 cells stained with ab52947 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52947, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947) at 1/2000 dilution + fetal liver lysate at 10 µgSecondarygoat anti-rabbit HRP at 1/2000 dilution

ab52947 at 1/100-1/250 dilution staining Heme Oxygenase 1 in human liver by Immunohistochemistry, Paraffin embedded tissue.ab52947 at 1/100-1/250 dilution staining Heme Oxygenase 1 in human liver by Immunohistochemistry, Paraffin embedded tissue.

Product References

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