Anti-Heme Oxygenase 1 antibody [GTS-1]
| Name | Anti-Heme Oxygenase 1 antibody [GTS-1] |
|---|---|
| Supplier | Abcam |
| Catalog | ab12220 |
| Prices | $384.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | GTS-1 |
| Applications | FC ELISA WB IHC-P IHC-F B/N |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Rat heme oxygenase 1 |
| Description | Mouse Monoclonal |
| Gene | HMOX1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HEK293 cells stained with ab12220 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12220, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/29824_Heme-Oxygenase-1-Primary-antibodies-ab12220-1.jpg)
Overlay histogram showing HEK293 cells stained with ab12220 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12220, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
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Yang H, Li H, Wang Z, Shi Y, Jiang G, Zeng F. J Surg Res. 2013 Dec;185(2):825-32.
In vivo nanoneurotoxicity screening using oxidative stress and neuroinflammation - In vivo nanoneurotoxicity screening using oxidative stress and neuroinflammation
Kim Y, Kong SD, Chen LH, Pisanic TR 2nd, Jin S, Shubayev VI. Nanomedicine. 2013 Oct;9(7):1057-66.
Molecular insights into beta-galactoside alpha2,6-sialyltransferase secretion in - Molecular insights into beta-galactoside alpha2,6-sialyltransferase secretion in
Kitazume S, Oka R, Ogawa K, Futakawa S, Hagiwara Y, Takikawa H, Kato M, Kasahara A, Miyoshi E, Taniguchi N, Hashimoto Y. Glycobiology. 2009 May;19(5):479-87.