Anti-Hephaestin antibody
| Name | Anti-Hephaestin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab56729 |
| Prices | $401.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Applications | WB IHC-F ICC/IF ICC/IF IHC-F |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Recombinant fragment, corresponding to amino acids 315-425 of Human Hephaestin |
| Description | Mouse Monoclonal |
| Gene | HEPH |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab56729 staining hephaestin (red) in Rat brain capillary endothelial cell tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 1.5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/200 in PBS + 0.3% Triton X-100) for 12 hours at 4°C. A Cy3®-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.See Abreview
ICC/IF image of hepaestin staining on rat P3 glial culture using ab56729. The cells were fixed with 4% PFA in 0.1M PBS. Mature astrocytes are visible in red. Nucleus stained with Hoechst at high power magnification.See Abreview
All lanes : Anti-Hephaestin antibody (ab56729) at 1/1000 dilutionLane 1 : Whole tissue lysate prepared from murine liverLane 2 : Whole tissue lysate prepared from murine liverLysates/proteins at 20 µg per lane.SecondaryHRP conjugated sheep anti-mouse polyclonal at 1/5000 dilutiondeveloped using the ECL technique
Product References
Ferroportin and exocytoplasmic ferroxidase activity are required for brain - Ferroportin and exocytoplasmic ferroxidase activity are required for brain
McCarthy RC, Kosman DJ. J Biol Chem. 2013 Jun 14;288(24):17932-40.
Transient expression of iron transport proteins in the capillary of the - Transient expression of iron transport proteins in the capillary of the
Yang WM, Jung KJ, Lee MO, Lee YS, Lee YH, Nakagawa S, Niwa M, Cho SS, Kim DW. Cell Mol Neurobiol. 2011 Jan;31(1):93-9.