Anti-HES5 antibody [EPR15578] (ab194111) at 1/10000 dilution + 293 cell lysate at 10 µgSecondaryGoat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Anti-HES5 antibody [EPR15578] (ab194111) at 1/10000 dilution + Human cerebellum lysate at 10 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Anti-HES5 antibody [EPR15578] (ab194111) at 1/2000 dilution + Human fetal brain lysate at 20 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
All lanes : Anti-HES5 antibody [EPR15578] (ab194111) at 1/2000 dilutionLane 1 : Mouse brain lysateLane 2 : Mouse kidney lysateLane 3 : Mouse spleen lysateLane 4 : Rat brain lysateLane 5 : Rat kidney lysateLane 6 : Rat spleen lysateLane 7 : C6 cell lysateLane 8 : PC-12 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human glioblastoma tissue labeling HES5 with ab194111 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
Immunohistochemical analysis of paraffin-embedded Human adenocarcinoma of endometrium tissue labeling HES5 with ab194111 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling HES5 with ab194111 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
Western blot detection of HES5 in Human cerebellum cell lysate immunoprecipitated using ab194111 at 1/40 dilution.Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.Blocking/Dilution buffer: 5% NFDM/TBST.