Anti-HEXIM1 antibody - ChIP Grade

• Supplier: Abcam
• Catalog: ab25388
• Prices: $403.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ChIP WB ICC/IF ICC/IF IHC-P IP
• Species Reactivities: Mouse, Rat, Human, Dog
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human HEXIM1
• Description: Rabbit Polyclonal
• Gene: HEXIM1
• Conjugate: Unconjugated

Product images

All lanes : Anti-HEXIM1 antibody - ChIP Grade (ab25388) at 1/250 dilutionLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell LysateLane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 5 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

HEXIM1 - ChIP Grade was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to HEXIM1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab25388.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 54kD: HEXIM1 .

Image courtesy of Human Protein Atlasab25388 staining HEXIM1 in human kidney. Paraffin embedded human kidney tissue was incubated with ab25388 (1/100 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab25388 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

ICC/IF image of ab25388 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab25388 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

Release of positive transcription elongation factor b (P-TEFb) from 7SK small - Release of positive transcription elongation factor b (P-TEFb) from 7SK small

Liu P, Xiang Y, Fujinaga K, Bartholomeeusen K, Nilson KA, Price DH, Peterlin BM. J Biol Chem. 2014 Apr 4;289(14):9918-25.

Acute hypoxia affects P-TEFb through HDAC3 and HEXIM1-dependent mechanism to - Acute hypoxia affects P-TEFb through HDAC3 and HEXIM1-dependent mechanism to

Safronova OS, Nakahama K, Morita I. Nucleic Acids Res. 2014 Aug;42(14):8954-69.

SERPINE1 and SMA expression at the invasive front predict extracapsular spread - SERPINE1 and SMA expression at the invasive front predict extracapsular spread

Dhanda J, Triantafyllou A, Liloglou T, Kalirai H, Lloyd B, Hanlon R, Shaw RJ, Sibson DR, Risk JM. Br J Cancer. 2014 Nov 25;111(11):2114-21.

Histone deacetylase inhibitors (HDACis) that release the positive transcription - Histone deacetylase inhibitors (HDACis) that release the positive transcription

Bartholomeeusen K, Fujinaga K, Xiang Y, Peterlin BM. J Biol Chem. 2013 May 17;288(20):14400-7.

CTIP2 is a negative regulator of P-TEFb. - CTIP2 is a negative regulator of P-TEFb.

Cherrier T, Le Douce V, Eilebrecht S, Riclet R, Marban C, Dequiedt F, Goumon Y, Paillart JC, Mericskay M, Parlakian A, Bausero P, Abbas W, Herbein G, Kurdistani SK, Grana X, Van Driessche B, Schwartz C, Candolfi E, Benecke AG, Van Lint C, Rohr O. Proc Natl Acad Sci U S A. 2013 Jul 30;110(31):12655-60. doi:

Bromodomain and extra-terminal (BET) bromodomain inhibition activate - Bromodomain and extra-terminal (BET) bromodomain inhibition activate

Bartholomeeusen K, Xiang Y, Fujinaga K, Peterlin BM. J Biol Chem. 2012 Oct 19;287(43):36609-16.

The leukemogenic AF4-MLL fusion protein causes P-TEFb kinase activation and - The leukemogenic AF4-MLL fusion protein causes P-TEFb kinase activation and

Benedikt A, Baltruschat S, Scholz B, Bursen A, Arrey TN, Meyer B, Varagnolo L, Muller AM, Karas M, Dingermann T, Marschalek R. Leukemia. 2011 Jan;25(1):135-44.

AFF4, a component of the ELL/P-TEFb elongation complex and a shared subunit of - AFF4, a component of the ELL/P-TEFb elongation complex and a shared subunit of

Lin C, Smith ER, Takahashi H, Lai KC, Martin-Brown S, Florens L, Washburn MP, Conaway JW, Conaway RC, Shilatifard A. Mol Cell. 2010 Feb 12;37(3):429-37.

Identification of the P-TEFb complex-interacting domain of Brd4 as an inhibitor - Identification of the P-TEFb complex-interacting domain of Brd4 as an inhibitor

Urano E, Kariya Y, Futahashi Y, Ichikawa R, Hamatake M, Fukazawa H, Morikawa Y, Yoshida T, Koyanagi Y, Yamamoto N, Komano J. FEBS Lett. 2008 Dec 10;582(29):4053-8.

Cross-talk of GATA-1 and P-TEFb in megakaryocyte differentiation. - Cross-talk of GATA-1 and P-TEFb in megakaryocyte differentiation.

Elagib KE, Mihaylov IS, Delehanty LL, Bullock GC, Ouma KD, Caronia JF, Gonias SL, Goldfarb AN. Blood. 2008 Dec 15;112(13):4884-94.