Anti-HFE antibody
| Name | Anti-HFE antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab55182 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Recombinant fragment: SHTLQVILGC EMQEDNSTEG YWKYGYDGQD HLEFCPDTLD WRAAEPRAWP TKLEWERHKI RARQNRAYLE RDCPAQLQQL LELGRGVLDQ Q, corresponding to amino acids 115-206 of Human HFE Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | HFE |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of HFE staining (ab55182) in human blood film. The sections were incubated in 0.3% Triton X with 0.1M PBS to permeabilise the cells and in 10% serum for 30 minutes at RT to block non-specific protein-protein interactions. The sections were then incubated with ab55182 (1:500) for 4 hrs at RT, followed by Alexa 568 conjugated secondary antibody. Red staining of the cell membrane was observed. Macrophages were stained using a CD68 primary and Alexa Fluor 488 conjugated secondary antibody. The nucleus was visualised using Hoechst.See Abreview
Western blot against tagged recombinant protein immunogen using ab55182 HFE antibody at 1ug/ml.
ab55182 at 10 ug/ml staining HFE in human Hela cells by Immunocytochemistry/ Immunofluorescence.