Lanes 1 - 2 : Anti-HFE antibody [EPR6751(2)] (ab133639) at 1/1000 dilutionLanes 3 - 4 : Rabbit monoclonal anti-myc tagLane 1 : HepG2 whole cell lysate - untransfectedLane 2 : HepG2 whole cell lysate - transfected with pCMV6 human HFE Tag Flag MycLane 3 : HepG2 whole cell lysate - untransfectedLane 4 : HepG2 whole cell lysate - transfected with pCMV6 human HFE Tag Flag MycLysates/proteins at 25 µg per lane.SecondaryHRP-conjugated anti-rabbit IgG polyclonal at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
All lanes : Anti-HFE antibody [EPR6751(2)] (ab133639) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : A375 cell lysateLane 3 : Caco-2 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution
Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD