Anti-HGS antibody
| Name | Anti-HGS antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab56468 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | WB IHC-P ICC/IF ICC/IF FC |
| Species Reactivities | Rat, Human |
| Antigen | Recombinant fragment: KLEIMRQKKQ EYLEVQRQLA IQRLQEQEKE RQMRLEQQKQ TVQMRAQMPA FPLPYAQLQA MPAAGGVLYQ PSGPASFPST FSPAGSVEGS PMHGVYMSQP , corresponding to amino acids 513-612 of Human HGS Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | HGS |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of HGS staining in Human normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab56468, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
![Overlay histogram showing Jurkat cells stained with ab56468 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56468, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/373_HGS-Primary-antibodies-ab56468-1.jpg)
Overlay histogram showing Jurkat cells stained with ab56468 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56468, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
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Multivesicular bodies associate with components of miRNA effector complexes and - Multivesicular bodies associate with components of miRNA effector complexes and
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Proteomics study of medullary thyroid carcinomas expressing RET germ-line - Proteomics study of medullary thyroid carcinomas expressing RET germ-line
Gorla L, Mondellini P, Cuccuru G, Micciche F, Cassinelli G, Cremona M, Pierotti MA, Lanzi C, Bongarzone I. Mol Carcinog. 2009 Mar;48(3):220-31.