HGS

Gene Symbol HGS
Entrez Gene 9146
Alt Symbol HRS
Species Human
Gene Type protein-coding
Description hepatocyte growth factor-regulated tyrosine kinase substrate
Other Description human growth factor-regulated tyrosine kinase substrate|protein pp110
Swissprots O14964 Q9NR36
Accessions EAW89675 EAW89676 EAW89677 EAW89678 EAW89679 O14964 AF260566 AAF82361 AI365104 AK021483 AK097197 AK294190 BAG57506 AK303800 BAG64754 BC003565 AAH03565 BT009754 AAP88756 D84064 BAA23366 DQ892551 ABM83477 DQ895762 ABM86688 DR001213 U43895 AAC51929 XM_005257794 XP_005257851 XM_011525463 XP_011523765 XM_011525464 XP_011523766 NM_004712 NP_004703
Function Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down- regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation
Subcellular Location Cytoplasm. Early endosome membrane; Peripheral membrane protein; Cytoplasmic side. Endosome, multivesicular body membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
Tissue Specificity Ubiquitous expression in adult and fetal tissues with higher expression in testis and peripheral blood leukocytes. {ECO:0000269|PubMed:9407053, ECO:0000269|PubMed:9630564}.
Top Pathways Endocytosis, Phagosome

HRS Antibody - 14346 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using HRS Antibody.

Hrs (C-7) - sc-271455 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Hrs (C-7): sc-271455. Western blot analysis of Hrs expression in K-562 (A) and HEL 92.1.7 (B) whole cell lysates.

Hrs (D-3) - sc-271925 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Hrs (D-3): sc-271925. Western blot analysis of Hrs expression in HEL 92.1.7 whole cell lysate.

Hrs (M-79) - sc-30221 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Hrs (M-79): sc-30221. Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.

Hrs (D-11) - sc-271160 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Hrs (D-11): sc-271160. Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of glandular cells.

Hrs (D-6) - sc-166843 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Hrs (D-6): sc-166843. Western blot analysis of Hrs expression in non-transfected: sc-117752 (A) and mouse Hrs transfected: sc-125474 (B) 293T whole cell lysates.

Hrs (V-20) - sc-23791 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Hrs (V-20): sc-23791. Western blot analysis of Hrs expression in CCRF-CEM (A), AML-193 (B) and HEL 92.1.7 (C) whole cell lysates.

Anti-HGS (phospho Y334) antibody - ab62447 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry analysis of HGS in human brain tissue (paraffin section) using ab62447 (1/50); in the presence or absence of phosphopeptide.

Anti-HGS antibody - ab56468 from Abcam

Prices $390.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
IHC image of HGS staining in Human normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab56468, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-HGS antibody - ab70180 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
HUVEC cells with ab70180 at 1/500 dilution, with and without the immunising peptide.

Anti-HGS antibody - ab61194 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
1/50-1/100 ab61194 staining total HRS on human brain tissue sections. Samples were treated -/+ peptide.

Anti-HGS antibody - ab190679 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-HGS antibody (ab190679) at 1/500 dilution + Mouse eye extracts at 30 µg

Anti-HGS antibody - ab72521 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunoprecipitation/ Western Blot of HGS. Lane 1: ab72521 at 3µg/mg Hela whole cell lysate (1mg for IP, 20% of IP loaded). Lane 2: Control IgG. ab72521 at 1µg/ml for WB. Chemiluminescence with an exposure time of 30 seconds.

Anti-HGS antibody [19-5] - ab150653 from Abcam

Prices $378.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 19-5
All lanes : Anti-HGS antibody [19-5] (ab150653) at 1/1000 dilutionLane 1 : Human brain lysateLane 2 : HeLa cell lysate

Anti-HGS antibody [3H7] - ab156796 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3H7
All lanes : Anti-HGS antibody [3H7] (ab156796) at 1/4000 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNALane 2 : HEK293T cells transfected with pCMV6-ENTRY HGS cDNALysates/proteins at 5 µg per lane.

Anti-HGS antibody - C-terminal - ab155539 from Abcam

Prices $392.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab155539 immunoprecipitating HGS in human hTERT-RPE1 whole cell lysate. Cell lysate from 4000000 cells was incubated with primary antibody (1/500 in triton 1% buffer) for 12 hours at 4°C. Western blotting confirmed successful immunoprecipation. Lower 50kDa band corresponds to IgG.See Abreview

Anti-HGS antibody - N-terminal - ab135511 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-HGS antibody - N-terminal (ab135511) at 1/100 dilution + Mouse spleen tissue lysate at 35 µg

Anti-HRS / HGS Antibody - LS-C36820 from LifeSpan Bioscience

Prices $445.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal

Anti-HRS / HGS Antibody (aa32-49) - LS-C149014 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-HRS / HGS Antibody (aa513-612, clone 10D205, Azide-free) - LS-C183697 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone 10D205

Anti-HRS / HGS Antibody (aa331-344) - LS-C54980 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Antibody (2.5 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Anti-HRS / HGS Antibody (clone 1C2) - LS-C174537 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1C2
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HGS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HGS.

Anti-HRS / HGS Antibody (phospho-Tyr216) - LS-C178065 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of p-Hrs (Y216) pAb in extracts from A549 cells.

Anti-HRS / HGS Antibody (N-Terminus, AP) - LS-C278247 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HRS / HGS Antibody (N-Terminus, APC) - LS-C278248 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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