Anti-HIF1AN antibody
| Name | Anti-HIF1AN antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab12289 |
| Prices | $380.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F ICC/IF ICC/IF WB |
| Species Reactivities | Chicken, Human |
| Antigen | Synthetic peptide derived from within residues 250 to the C-terminus of Human FIH/asparagine hydroxylase |
| Description | Rabbit Polyclonal |
| Gene | HIF1AN |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-HIF1AN antibody (ab12289) at 1/500 dilutionLane 1 : Hela nuclear extractLane 2 : Hela whole cell lysateLane 3 : A431 whole cell lysateLane 4 : Jurkat whole cell lysateLane 5 : Hek293 cell lysateLane 6 : Hela nuclear extract with FIH blocking peptide at 1 µg/mlLane 7 : Hela whole cell lysateLane 8 : A431 whole cell lysate with FIH blocking peptide at 1 µg/mlLane 9 : Jurkat whole cell lysate with FIH blocking peptide at 1 µg/mlLane 10 : Hek293 whole cell lysate with FIH blocking peptide at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to rabbit IgG H&L (HRP) at 1/5000 dilution
ICC/IF image of ab12289 stained human Hek293 cells. The cells were 4% PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab12289, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Product References
Activation of negative regulators of the hypoxia-inducible factor (HIF) pathway - Activation of negative regulators of the hypoxia-inducible factor (HIF) pathway
Zolk O, Solbach TF, Eschenhagen T, Weidemann A, Fromm MF. Biochem Biophys Res Commun. 2008 Nov 14;376(2):315-20. doi:
Interaction with factor inhibiting HIF-1 defines an additional mode of - Interaction with factor inhibiting HIF-1 defines an additional mode of
Zheng X, Linke S, Dias JM, Zheng X, Gradin K, Wallis TP, Hamilton BR, Gustafsson M, Ruas JL, Wilkins S, Bilton RL, Brismar K, Whitelaw ML, Pereira T, Gorman JJ, Ericson J, Peet DJ, Lendahl U, Poellinger L. Proc Natl Acad Sci U S A. 2008 Mar 4;105(9):3368-73. doi: