HIF1AN

Gene Symbol HIF1AN
Entrez Gene 55662
Alt Symbol FIH1
Species Human
Gene Type protein-coding
Description hypoxia inducible factor 1, alpha subunit inhibitor
Other Description FIH-1|factor inhibiting HIF-1|factor inhibiting HIF1|hypoxia-inducible factor 1-alpha inhibitor|hypoxia-inducible factor asparagine hydroxylase|peptide-aspartate beta-dioxygenase
Swissprots Q969Q7 D3DR69 Q9NPV5 Q9NWT6 Q5W147
Accessions CDM55393 CDM55574 EAW49817 EAW49818 Q9NWT6 AF395830 AAL27308 AK000622 BAA91291 AK025680 BAB15213 AK295353 BAG58320 AL359615 CAB94885 AW015561 BC007719 AAH07719 BC015574 DQ892767 ABM83693 DQ896013 ABM87012 XM_011539940 XP_011538242 XM_011539941 XP_011538243 NM_017902 NP_060372
Function Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300- interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation. {ECO:0000269|PubMed:12042299, ECO:0000269|PubMed:12080085, ECO:0000269|PubMed:17003112, ECO:0000269|PubMed:17573339, ECO:0000269|PubMed:18299578, ECO:0000269|PubMed:19245366, ECO:0000269|PubMed:21177872, ECO:0000269|PubMed:21251231}.
Subcellular Location Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Note=Mainly cytoplasmic localization, but interaction with NOTCH1 results in nuclear localization and interaction with ABPA3 results in perinuclear localization in macrophages.

FIH (D19B3) Rabbit mAb - 4426 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D19B3
Western blot analysis of extracts from various cell types using FIH (D19B3) Rabbit mAb.

FIH-1 (A-5) - sc-271780 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
FIH-1 (A-5): sc-271780. Western blot analysis of FIH-1 expression in Jurkat whole cell lysate.

FIH-1 (D-12) - sc-365016 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
FIH-1 (D-12): sc-365016. Western blot analysis of FIH-1 expression in Jurkat whole cell lysate.

FIH-1 (F-11) - sc-365128 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
FIH-1 (F-11): sc-365128. Western blot analysis of FIH-1 expression in Jurkat whole cell lysate.

FIH-1 (H-229) - sc-48813 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

FIH-1 (N-18) - sc-26219 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
FIH-1 (N-18): sc-26219. Western blot analysis of FIH-1 expression in Sol8 nuclear extract (A) and rat skeletal muscle tissue extract (B).

Anti-HIF1AN antibody - ab187524 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid cancer tissue labelling HIF1AN with ab187524 at 1/200. Magnification: 400x.

Anti-HIF1AN antibody - ab12289 from Abcam

Prices $380.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-HIF1AN antibody (ab12289) at 1/500 dilutionLane 1 : Hela nuclear extractLane 2 : Hela whole cell lysateLane 3 : A431 whole cell lysateLane 4 : Jurkat whole cell lysateLane 5 : Hek293 cell lysateLane 6 : Hela nuclear extract with FIH blocking peptide at 1 µg/mlLane 7 : Hela whole cell lysateLane 8 : A431 whole cell lysate with FIH blocking peptide at 1 µg/mlLane 9 : Jurkat whole cell lysate with FIH blocking peptide at 1 µg/mlLane 10 : Hek293 whole cell lysate with FIH blocking peptide at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to rabbit IgG H&L (HRP) at 1/5000 dilution

Anti-HIF1AN antibody [EPR3658] - ab92498 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3658
All lanes : Anti-HIF1AN antibody [EPR3658] (ab92498) at 1/5000 dilutionLane 1 : Jurkat cell lysateLane 2 : 293T cell lysateLane 3 : A375 cell lysateLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit HRP at 1/2000 dilution

Anti-HIF1AN antibody [EPR3659] - ab92304 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3659
Overlay histogram showing Jurkat cells stained with ab92304 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92304, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-HIF1AN antibody [FIH162C] - ab103401 from Abcam

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone FIH162C
Overlay histogram showing Jurkat cells stained with ab103401 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab103401, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-HIF1AN antibody - ab63163 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-HIF1AN antibody (ab63163) at 1/250 dilutionLane 1 : A375 cell lysateLane 2 : Mouse brain lysate

Anti-HIF1AN antibody - ab50931 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Lane 1 : Molecular weight markersLane 2 : Anti-HIF1AN antibody (ab50931) at 0.25 µg/mlLane 1 : As aboveLane 2 : Jurkat cell lysate at 10 µgSecondaryLane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-HIF1AN antibody - ab57601 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-HIF1AN antibody - ab56044 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-HIF1AN antibody - Aminoterminal end - ab56045 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-HIF1AN Antibody (C-Terminus, APC) - LS-C244647 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HIF1AN Antibody - LS-C308237 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-HIF1AN Antibody (clone 162c) - LS-C143194 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 162c
Factor Inhibiting HIF-1 Antibody (162c) - IHC of FIH in human renal cancer using DAB with hematoxylin counterstain.

Anti-HIF1AN Antibody (C-Terminus, PE) - LS-C244649 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HIF1AN Antibody (C-Terminus) - LS-C327559 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HIF1AN Antibody (N-Terminus) - LS-C327561 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HIF1AN Antibody (C-Terminus) IHC-plus™ - LS-B667 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-HIF1AN antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B667 concentration 5 ug/ml.

Anti-HIF1AN Antibody - LS-C341625 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-HIF1AN Antibody (Internal, clone EPR3658) - LS-C105594 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3658
IHC of paraffin-embedded human brain tissue using anti-FIH1 antibody (LS-C105594).
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