Anti-HIF1AN antibody [FIH162C]
| Name | Anti-HIF1AN antibody [FIH162C] |
|---|---|
| Supplier | Abcam |
| Catalog | ab103401 |
| Prices | $359.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | FIH162C |
| Applications | WB IHC-P FC |
| Species Reactivities | Human |
| Antigen | Full length human FIH expressed in Escherichia coli BL21(DE3) cells |
| Description | Mouse Monoclonal |
| Gene | HIF1AN |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing Jurkat cells stained with ab103401 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab103401, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/678_HIF1AN-Primary-antibodies-ab103401-1.jpg)
Overlay histogram showing Jurkat cells stained with ab103401 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab103401, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
Use of novel monoclonal antibodies to determine the expression and distribution - Use of novel monoclonal antibodies to determine the expression and distribution
Soilleux EJ, Turley H, Tian YM, Pugh CW, Gatter KC, Harris AL. Histopathology. 2005 Dec;47(6):602-10.
Genetic analysis of the role of the asparaginyl hydroxylase factor inhibiting - Genetic analysis of the role of the asparaginyl hydroxylase factor inhibiting
Stolze IP, Tian YM, Appelhoff RJ, Turley H, Wykoff CC, Gleadle JM, Ratcliffe PJ. J Biol Chem. 2004 Oct 8;279(41):42719-25. Epub 2004 Aug 9.