All lanes : Anti-HIP55 antibody - N-terminal (ab154016) at 1/1500 dilutionLane 1 : HeLa S3 whole cell lysateLane 2 : MOLT4 whole cell lysateLysates/proteins at 30 µg per lane.
Immunohistochemical analysis of paraffin-embedded Human H1299 xenograft tissue labeling HIP55 with ab154016 at 1/100 dilution.
Immunofluorescent analysis of HeLa cells (fixed in iced-cold Methanol for 5 min) labeling HIP55 with ab154016 at 1/500 dilution (green). Right: Blue staining represents cells costained with Hoechst 33343.