DBNL

Gene Symbol DBNL
Entrez Gene 28988
Alt Symbol ABP1, HIP-55, HIP55, SH3P7
Species Human
Gene Type protein-coding
Description drebrin-like
Other Description HPK1-interacting protein of 55 kDa|SH3 domain-containing protein 7|cervical SH3P7|cervical mucin-associated protein|drebrin-F|drebrin-like protein|src homology 3 domain-containing protein HIP-55
Swissprots Q96K74 Q6IAI8 P84070 C9J7P1 Q9NR72 B4DDP6 Q9HBN8 Q96F30 Q9UJU6 A4D2I9 B4DEM2
Accessions EAL23770 EAW61129 EAW61130 EAW61131 EAW61132 Q9UJU6 AB209486 BAD92723 AF077353 AAF80228 AF151364 AAG13120 AF197060 AAF13701 AF218020 AAG17262 AF250287 AAF81273 AK027367 BAB55065 AK126142 AK293151 BAG56697 AK293279 BAG56807 AK293340 BAG56856 AK293698 BAG57133 AK296778 BAG59356 AK298102 BAG60387 AK300630 BAG62321 AK302037 BAG63431 BC011677 AAH11677 BC023589 BC031687 AAH31687 CR457167 CAG33448 DC404852 HQ447892 ADQ32376 JF432143 ADZ15360 XM_011515325 XP_011513627 NM_001014436 NP_001014436 NM_001122956 NP_001116428 NM_001284313 NP_001271242 NM_001284315 NP_001271244 NM_014063 NP_054782
Function Adapter protein that binds F-actin and DNM1, and thereby plays a role in receptor-mediated endocytosis. Plays a role in the reorganization of the actin cytoskeleton, formation of cell projections, such as neurites, in neuron morphogenesis and synapse formation via its interaction with WASL and COBL. Does not bind G- actin and promote actin polymerization by itself. Required for the formation of organized podosome rosettes (By similarity). May act as a common effector of antigen receptor-signaling pathways in leukocytes. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes. {ECO:0000250, ECO:0000269|PubMed:14729663}.
Subcellular Location Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Cell projection, ruffle {ECO:0000250}. Cytoplasm, cell cortex {ECO:0000250}. Cytoplasm, cytosol {ECO:0000250}. Cell junction, synapse {ECO:0000250}. Cell projection {ECO:0000250}. Cell membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}. Cytoplasmic vesicle, clathrin-coated vesicle membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}. Golgi apparatus membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}. Cell projection, podosome {ECO:0000250}. Note=Detected in neuron cell body and cell projections, such as neurites. Colocalizes with cytosolic dynamin in hippocampus neurons (By similarity). Cortical actin cytoskeleton. Associates with lamellipodial actin and membrane ruffles. Colocalizes with actin and cortactin at podosome dots and podosome rosettes (By similarity). {ECO:0000250}.

HIP-55 (7) - sc-135935 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/500 µl
Host Mouse
Clonality Monoclonal
HIP-55 (7): sc-135935. Western blot analysis of HIP-55 expression in HeLa whole cell lysate.

HIP-55 (E-5) - sc-398358 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
HIP-55 (E-5): sc-398358. Western blot analysis of HIP-55 expression in non-transfected 293T: sc-117752 (A), human HIP-55 transfected 293T: sc-371066 (B) and Raji (C) whole cell lysates.

HIP-55 (E-11) - sc-398498 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
HIP-55 (E-11): sc-398498. Western blot analysis of HIP-55 expression in non-transfected 293T: sc-117752 (A), human HIP-55 transfected 293T: sc-371066 (B), U-698-M (C), Jurkat (D) and Raji (E) whole cell lysates.

HIP-55 (F-9) - sc-398351 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
HIP-55 (F-9): sc-398351. Western blot analysis of HIP-55 expression in non-transfected 293T: sc-117752 (A), human HIP-55 transfected 293T: sc-371066 (B), U-698-M (C), Jurkat (D) and Raji (E) whole cell lysates.

HIP-55 (H-87) - sc-366772 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
HIP-55 (H-87): sc-366772. Western blot analysis of HIP-55 expression in non-transfected 293T: sc-117752 (A), human HIP-55 transfected 293T: sc-371066 (B), U-698-M (C), Jurkat (D) and NTERA-2 cl.D1 (E) whole cell lysates.

HIP-55 (N-17) - sc-79973 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
HIP-55 (N-17): sc-79973. Western blot analysis of HIP-55 expression in non-transfected: sc-117752 (A) and human HIP-55 transfected: sc-371066 (B) 293T whole cell lysates.

HIP-55 (V-20) - sc-79975 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
HIP-55 (V-20): sc-79975. Western blot analysis of HIP-55 expression in non-transfected: sc-117752 (A) and human HIP-55 transfected: sc-371066 (B) 293T whole cell lysates.

Anti-HIP55 antibody - ab77147 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-HIP55 antibody (ab77147) at 1 µg/mlLane 1 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell LysateLane 2 : SK N BE (Human neuroblastoma) Whole Cell LysateLane 3 : SK N SH (Human neuroblastoma) Whole Cell LysateLane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-HIP55 antibody - ab2836 from Abcam

Prices $370.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
ab2836 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence.

Anti-HIP55 antibody - ab99113 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-HIP55 antibody (ab99113) at 1 µg/ml + Human fetal muscle lysate at 10 µg

Anti-HIP55 antibody - ab118794 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Detection of HIP55 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab118794 at 6 µg/mg lysate for IP. For WB detection an anti-HIP55 antibody which recognizes an upstream epitope was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Anti-HIP55 antibody [M334] - ab86708 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone M334
All lanes : Anti-HIP55 antibody [M334] (ab86708) at 1/500 dilutionLane 1 : Jurkat cell lysateLane 2 : HeLa cell lysateLane 3 : Mouse brain lysateLane 4 : A431 cell lysateObserved band size : 55 kDa (why is the actual band size different from the predicted?)

Anti-HIP55 antibody - N-terminal - ab154016 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-HIP55 antibody - N-terminal (ab154016) at 1/1500 dilutionLane 1 : HeLa S3 whole cell lysateLane 2 : MOLT4 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-SH3P7 / HIP-55 Antibody (aa292-319, APC) - LS-C255867 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SH3P7 / HIP-55 Antibody (aa292-319, AP) - LS-C255868 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SH3P7 / HIP-55 Antibody (aa292-319, FITC) - LS-C255870 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SH3P7 / HIP-55 Antibody (aa292-319, Biotin) - LS-C255869 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SH3P7 / HIP-55 Antibody (aa292-319, Azide-free, HRP) - LS-C255871 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SH3P7 / HIP-55 Antibody (aa292-319, PE) - LS-C255873 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SH3P7 / HIP-55 Antibody (aa225-275) - LS-C290038 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-SH3P7 / HIP-55 Antibody (aa275-325) - LS-C290039 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-SH3P7 / HIP-55 Antibody (aa291-319) - LS-C320748 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SH3P7 / HIP-55 Antibody (aa251-300) - LS-C111559 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
DBNL / HIP-55 antibody LS-C111559 Western blot of Fetal Muscle lysate.

Anti-SH3P7 / HIP-55 Antibody (aa292-319) - LS-C167281 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
DBNL Antibody western blot of HL-60 cell line lysates (35 ug/lane). The DBNL antibody detected the DBNL protein (arrow).

Anti-SH3P7 / HIP-55 Antibody (clone 7B7) - LS-C339559 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 7B7
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DBNL (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DBNL.
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