Anti-HIPK2 antibody
| Name | Anti-HIPK2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab28507 |
| Prices | $401.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF ELISA WB IHC-P IHC-F ChIP |
| Species Reactivities | Human, Mouse, Rat, Bovine, Dog, Zebrafish |
| Antigen | Synthetic peptide derived from within residues 37 - 86 of human HIPK2 |
| Blocking Peptide | Human HIPK2 peptide |
| Description | Rabbit Polyclonal |
| Gene | HIPK2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pineal tissue labelling HIPK2 with ab28507 at 1/100. A Cy3-conjugated donkey anti-rabbit (1/200) was used as the secondary antibody. Positive staining seen the nuclei of pinealocytes and intersticial cells. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - HIPK2. Right - Merge.
ab28507, at 4.0-8.0ug/ml, staining human HIPK2 in (Human heart, cells with positive label are Myocardial cells) by Immunohistochemistry, Paraffin embedded tissue.
Anti-HIPK2 antibody (ab28507) at 0.06 µg/ml + HepG2 Cell LysateSecondaryRabbit Anti HIPK2 at 1/50000 dilution
ICC/IF image of ab28507 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28507, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Downregulation of HIPK2 increases resistance of bladder cancer cell to cisplatin - Downregulation of HIPK2 increases resistance of bladder cancer cell to cisplatin
Lin J, Zhang Q, Lu Y, Xue W, Xu Y, Zhu Y, Hu X. PLoS One. 2014 May 20;9(5):e98418.
Transcriptional corepressors HIPK1 and HIPK2 control angiogenesis via - Transcriptional corepressors HIPK1 and HIPK2 control angiogenesis via
Shang Y, Doan CN, Arnold TD, Lee S, Tang AA, Reichardt LF, Huang EJ. PLoS Biol. 2013;11(4):e1001527.
Activation of the Wnt pathway through AR79, a GSK3beta inhibitor, promotes - Activation of the Wnt pathway through AR79, a GSK3beta inhibitor, promotes
Jiang Y, Dai J, Zhang H, Sottnik JL, Keller JM, Escott KJ, Sanganee HJ, Yao Z, McCauley LK, Keller ET. Mol Cancer Res. 2013 Dec;11(12):1597-610.