HIPK2

Gene Symbol HIPK2
Entrez Gene 28996
Alt Symbol PRO0593
Species Human
Gene Type protein-coding
Description homeodomain interacting protein kinase 2
Other Description hHIPk2|homeodomain-interacting protein kinase 2
Swissprots Q9H2Y1 Q75MR7 Q9H2X6 Q8WWI4
Accessions AAS00368 CAI38625 CAI40576 EAL24035 EAW83925 EAW83926 EAW83927 EAW83928 EAW83929 Q9H2X6 AF055019 AF111850 AAF16689 AF207702 AAG35710 AF208291 AAG41236 AF326592 AAL37371 AI580907 AK074291 AL833223 BC041926 BC043184 BC043652 BC045719 BC168360 AAI68360 BX647377 CK818532 CN413736 XM_006715935 XP_006715998 XM_011516077 XP_011514379 XM_011516078 XP_011514380 XM_011516079 XP_011514381 XM_011516080 XP_011514382 XM_011516081 XP_011514383 NM_001113239 NP_001106710 NM_022740 NP_073577
Function Serine/threonine-protein kinase involved in transcription regulation, p53/TP53-mediated cellular apoptosis and regulation of the cell cycle. Acts as a corepressor of several transcription factors, including SMAD1 and POU4F1/Brn3a and probably NK homeodomain transcription factors. Phosphorylates PDX1, ATF1, PML, p53/TP53, CREB1, CTBP1, CBX4, RUNX1, EP300, CTNNB1, HMGA1 and ZBTB4. Inhibits cell growth and promotes apoptosis through the activation of p53/TP53 both at the transcription level and at the protein level (by phosphorylation and indirect acetylation). The phosphorylation of p53/TP53 may be mediated by a p53/TP53-HIPK2-AXIN1 complex. Involved in the response to hypoxia by acting as a transcriptional co-suppressor of HIF1A. Mediates transcriptional activation of TP73. In response to TGFB, cooperates with DAXX to activate JNK. Negative regulator through phosphorylation and subsequent proteasomal degradation of CTNNB1 and the antiapoptotic factor CTBP1. In the Wnt/beta-catenin signa
Subcellular Location Nucleus, PML body. Cytoplasm. Note=Concentrated in PML/POD/ND10 nuclear bodies. Small amounts are cytoplasmic.
Tissue Specificity Highly expressed in heart, muscle and kidney. Weakly expressed in a ubiquitous way. Down-regulated in several thyroid and breast tumors. {ECO:0000269|PubMed:11267674, ECO:0000269|PubMed:11798164}.

HIPK2 Antibody - 5091 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a mouse HIPK2 construct (+), using HIPK2 Antibody.

HIPK2 (P-17) - sc-10293 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

HIPK2 (C-15) - sc-10294 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

HIPK2 (F-189) - sc-100383 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
HIPK2 (F-189): sc-100383. Western blot analysis of HIPK2 expression in MES-SA/Dx5 whole cell lysate.

Anti-HIPK2 antibody - ab28507 from Abcam

Prices $401.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pineal tissue labelling HIPK2 with ab28507 at 1/100. A Cy3-conjugated donkey anti-rabbit (1/200) was used as the secondary antibody. Positive staining seen the nuclei of pinealocytes and intersticial cells. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - HIPK2. Right - Merge.

Anti-HIPK2 antibody - ab57328 from Abcam

Host Mouse
Clonality Monoclonal
HIPK2 antibody (ab57328) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human esophagus.

Anti-HIPK2 antibody - ab75937 from Abcam

Prices $378.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-HIPK2 antibody (ab75937) at 1/200 dilution + Ramos cell line lysates at 35 µg

Anti-HIPK2 antibody - ab178505 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin tissue labeling HIPK2 with ab178505 at 10 µg/ml.

Anti-HIPK2 antibody [1F10] - ab117524 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 1F10
ab117524, at 5 µg/ml, staining HIPK2 in Human testis tissue by immunohistochemistry.

Anti-HIPK2 antibody [EPR3819] - ab108543 from Abcam

Prices $381.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3819
Overlay histogram showing HEK293 cells stained with ab108543 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108543, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-HIPK2 antibody [EPR3819] (Alexa Fluor® 488) - ab199208 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3819
ab199208 staining HIPK2 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

Anti-HIPK2 antibody [EPR3819] (Alexa Fluor® 647) - ab199491 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3819
ab199491 staining HIPK2 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

Anti-HIPK2 Antibody - LS-C129765 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-HIPK2 Antibody (Tyr361, AP) - LS-C244646 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HIPK2 Antibody (Tyr361, APC) - LS-C244650 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HIPK2 Antibody (Tyr361, Biotin) - LS-C244651 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HIPK2 Antibody (Tyr361, FITC) - LS-C244652 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HIPK2 Antibody (Tyr361, PE) - LS-C244653 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HIPK2 Antibody (C-Terminus, AP) - LS-C244654 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HIPK2 Antibody (C-Terminus, APC) - LS-C244655 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HIPK2 Antibody (C-Terminus, Biotin) - LS-C244656 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HIPK2 Antibody (C-Terminus, FITC) - LS-C244657 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HIPK2 Antibody (C-Terminus, PE) - LS-C244658 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HIPK2 Antibody (aa62-92, AP) - LS-C244659 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HIPK2 Antibody (aa62-92, Biotin) - LS-C244661 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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