Anti-HIPK2 antibody [EPR3819]
| Name | Anti-HIPK2 antibody [EPR3819] |
|---|---|
| Supplier | Abcam |
| Catalog | ab108543 |
| Prices | $381.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EPR3819 |
| Applications | WB IP ICC/IF FC |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic peptide corresponding to residues in Human HIPK2 |
| Description | Rabbit Monoclonal |
| Gene | HIPK2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Overlay histogram showing HEK293 cells stained with ab108543 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108543, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![All lanes : Anti-HIPK2 antibody [EPR3819] (ab108543) at 1/2000 dilutionLane 1 : HT-1080 cell lysateLane 2 : HepG2 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/801_HIPK2-Primary-antibodies-ab108543-1.JPG)
All lanes : Anti-HIPK2 antibody [EPR3819] (ab108543) at 1/2000 dilutionLane 1 : HT-1080 cell lysateLane 2 : HepG2 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution
Product References
Synthesis and properties of a selective inhibitor of homeodomain-interacting - Synthesis and properties of a selective inhibitor of homeodomain-interacting
Cozza G, Zanin S, Determann R, Ruzzene M, Kunick C, Pinna LA. PLoS One. 2014 Feb 24;9(2):e89176.
BRCA1-Dependent Translational Regulation in Breast Cancer Cells. - BRCA1-Dependent Translational Regulation in Breast Cancer Cells.
Dacheux E, Vincent A, Nazaret N, Combet C, Wierinckx A, Mazoyer S, Diaz JJ, Lachuer J, Venezia ND. PLoS One. 2013 Jun 21;8(6):e67313. Print 2013.