Anti-HIPPI antibody
| Name | Anti-HIPPI antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab5205 |
| Prices | $401.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF ChIP |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human HIPPI |
| Description | Rabbit Polyclonal |
| Gene | IFT57 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-HIPPI antibody (ab5205) at 1/1000 dilutionLane 1 : Recombinant human HIPPI at 10 µgLane 2 : Recombinant human HIPPI at 1 µgLane 3 : Recombinant human HIPPI at 0.1 µgLane 4 : yeast extract + AD-HIPPI fusion protein Lane 5 : yeast extract Lane 6 : human lymphoblastoid extract at 10 µgPerformed under reducing conditions.
ICC/IF image of ab5205 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab5205, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
All lanes : Anti-HIPPI antibody (ab5205) at 1 µg/mlLane 1 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 6 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902)Lane 7 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
All lanes : Anti-HIPPI antibody (ab5205) at 1 µg/mlLane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Lane 3 : Brain (Mouse) Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
Product References
Regulation of RE1 protein silencing transcription factor (REST) expression by - Regulation of RE1 protein silencing transcription factor (REST) expression by
Datta M, Bhattacharyya NP. J Biol Chem. 2011 Sep 30;286(39):33759-69.
Transcription regulation of caspase-1 by R393 of HIPPI and its molecular partner - Transcription regulation of caspase-1 by R393 of HIPPI and its molecular partner
Banerjee M, Datta M, Majumder P, Mukhopadhyay D, Bhattacharyya NP. Nucleic Acids Res. 2010 Jan;38(3):878-92.