Anti-HIRA antibody [EPR7416]

• Supplier: Abcam
• Catalog: ab129169
• Prices: $385.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR7416
• Applications: WB IP FC ICC/IF ICC/IF
• Species Reactivities: Human
• Antigen: Synthetic peptide corresponding to residues in Human HIRA
• Description: Rabbit Monoclonal
• Gene: HIRA
• Conjugate: Unconjugated

Product images

Overlay histogram showing HEK293 cells stained with ab129169 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab129169, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

All lanes : Anti-HIRA antibody [EPR7416] (ab129169) at 1/1000 dilutionLane 1 : 293T cell lysatesLane 2 : HeLa cell lysatesLane 3 : K562 cell lysatesLysates/proteins at 10 µg per lane.SecondaryHRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

ab129169, at 1/50 dilution, staining HIRA in HeLa cells by Immunofluorescence.

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD