HIRA

Gene Symbol HIRA
Entrez Gene 7290
Alt Symbol DGCR1, TUP1, TUPLE1
Species Human
Gene Type protein-coding
Description histone cell cycle regulator
Other Description DiGeorge critical region gene 1|HIR histone cell cycle regulation defective homolog A|TUP1-like enhancer of split protein 1|protein HIRA
Swissprots Q05BU9 Q8IXN2 P54198
Accessions CAA62800 EAX03043 EAX03044 P54198 AK289809 BAF82498 AK299950 BAG61778 AK302835 BAH13816 AK308642 BC032721 AAH32721 BC039835 AAH39835 CR456503 CAG30389 CU013101 CAK54532 CU013389 CAK54831 X75296 CAA53044 X77633 CAA54721 X81844 CAA57436 X89887 CAA61979 NM_003325 NP_003316
Function Cooperates with ASF1A to promote replication-independent chromatin assembly. Required for the periodic repression of histone gene transcription during the cell cycle. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit. {ECO:0000269|PubMed:12370293, ECO:0000269|PubMed:14718166, ECO:0000269|PubMed:15621527}.
Subcellular Location Nucleus. Nucleus, PML body. Note=Primarily, though not exclusively, localized to the nucleus. Localizes to PML bodies immediately prior to onset of senescence.
Tissue Specificity Expressed at high levels in kidney, pancreas and skeletal muscle and at lower levels in brain, heart, liver, lung, and placenta. {ECO:0000269|PubMed:9063745}.

HIRA (D6O8L) Rabbit mAb - 12463 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D6O8L
Western blot analysis of extracts from various cell lines using HIRA (D6O8L) Rabbit mAb.

HIRA (D2A5E) Rabbit mAb - 13307 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D2A5E
Western blot analysis of extracts from HCT 116, K-562, and COS-7 cells using HIRA (D2A5E) Rabbit mAb.

HIRA (H-300) - sc-48774 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
HIRA (H-300): sc-48774. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.

HIRA (N-20) - sc-18209 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

HIRA (374C6a) - sc-130636 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
HIRA (374C6a): sc-130636. Western blot analysis of human recombinant HIRA fusion protein.

Anti-HIRA (phospho T555) antibody - ab61095 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence analysis of HeLa cells, using ab61095 at a 1/500 dilution, minus immunising peptide (left image) and plus immunising peptide (right image).

Anti-HIRA antibody - ab20655 from Abcam

Prices $387.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-HIRA antibody (ab20655) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate (ab14660) at 10 µgSecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

Anti-HIRA antibody - ab111196 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab111196 at 1/50 dilution staining HIRA in paraffin-embedded Human Tonsil tissue by Immunohistochemistry. The image on the right is treated with the synthesized peptide.

Anti-HIRA antibody - ab130699 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-HIRA antibody (ab130699) at 1/100 dilution + K-562 cell line lysates at 35 µgdeveloped using the ECL technique

Anti-HIRA antibody [374C6a] - ab70734 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 374C6a
Anti-HIRA antibody [374C6a] (ab70734) + immunising recombinant protein

Anti-HIRA antibody [EPR7416] - ab129169 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7416
Overlay histogram showing HEK293 cells stained with ab129169 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab129169, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-HIRA Antibody - LS-C67869 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal

Anti-HIRA Antibody (aa32-61, PE) - LS-C258492 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HIRA Antibody (clone WC119.2H11) - LS-C137477 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone WC119.2H11

Anti-HIRA Antibody (aa32-61, AP) - LS-C259754 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HIRA Antibody (aa521-570) - LS-C117949 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded human tonsil tissue, using HIRA Antibody. The picture on the right is treated with the synthesized peptide.

Anti-HIRA Antibody (phospho-Thr555) - LS-C199057 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence of HeLa cells, using HIRA (Phospho-Thr555) Antibody. The sample on the right was incubated with synthetic peptide.

Anti-HIRA Antibody (aa32-61, APC) - LS-C259755 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HIRA Antibody (aa32-61, Biotin) - LS-C259756 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HIRA Antibody (aa601-650) - LS-C116044 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
HIRA antibody LS-C116044 Western blot of Mouse Brain lysate.

Anti-HIRA Antibody (aa32-61, FITC) - LS-C259757 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HIRA Antibody (aa32-61, Azide-free, HRP) - LS-C259758 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HIRA Antibody (clone 374C6a) - LS-C184651 from LifeSpan Bioscience

Prices $335.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 374C6a
Western blot of immunized recombinant protein using TUPLE1 antibody.

Anti-HIRA Antibody (aa31-61) - LS-C321957 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HIRA Antibody - LS-C308243 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
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