Anti-Histone H1 (phospho S1 + T3) antibody

• Supplier: Abcam
• Catalog: ab32979
• Prices: $242.00
• Sizes: 200 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Histone H1
• Description: Rabbit Polyclonal
• Gene: HIST1H1E
• Conjugate: Unconjugated

Product images

All lanes : Anti-Histone H1 (phospho S1 + T3) antibody (ab32979) at 1 µg/mlLane 1 : Calf Thymus Histone Preparation at 0.5 µgLane 2 : Calf Thymus Histone Preparation at 0.5 µg with Human Histone H1 (phospho S1 + T3) peptide (ab36684) at 1 µg/mlLane 3 : Calf Thymus Histone Preparation at 0.5 µg with Human Histone H1 peptide (ab36686) at 1 µg/mlLane 4 : HeLa Histone Preparation Nuclear Lysate - Colcemid-treated at 2.5 µgLane 5 : HeLa Histone Preparation Nuclear Lysate - Colcemid-treated at 2.5 µg with Human Histone H1 (phospho S1 + T3) peptide (ab36684) at 1 µg/mlLane 6 : HeLa Histone Preparation Nuclear Lysate - Colcemid-treated at 2.5 µg with Human Histone H1 peptide (ab36686) at 1 µg/mlSecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

This antibody gave a positive result in ELISA against the immunizing peptide ab36684 (blue line). It gave a negative result in ELISA against the non-modified equivalent peptide ab36686 (purple line). This indicates that it is specific for the modified peptide.