HIST1H1E

Gene Symbol	HIST1H1E
Entrez Gene	3008
Alt Symbol	H1.4, H1E, H1F4, H1s-4, dJ221C16.5
Species	Human
Gene Type	protein-coding
Description	histone cluster 1, H1e
Other Description	H1 histone family, member 4\|histone 1, H1e\|histone H1.4\|histone H1b\|histone H1s-4
Swissprots	Q4VB25 P10412
Accessions	AAA63187 AAN06702 EAW55534 P10412 AK313681 BAG36431 BC055095 BC096168 AAH96168 BC096169 AAH96169 BC096170 AAH96170 BC099632 AAH99632 EF065554 ABN72276 EF065555 ABN72277 NM_005321 NP_005312
Function	Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity). {ECO:0000250}.
Subcellular Location	Nucleus. Chromosome. Note=Mainly localizes in heterochromatin. Dysplays a punctuate staining pattern in the nucleus.

Histone H1 (H-2) - sc-393358 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

Histone H1 (H-2): sc-393358. Western blot analysis of Histone H1 expression in A-431 (A), LNCaP (B), HeLa (C) and HL-60 (D) whole cell lysates and Jurkat nuclear extract (E).

Histone H1 (G-1) - sc-393530 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

Histone H1 (G-1): sc-393530. Western blot analysis of Histone H1 expression in Jurkat nuclear extract (A) and LNCaP whole cell lysate (B).

Anti-Histone H1 (phospho ) antibody - ab4270 from Abcam

Prices	$384.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Rabbit polyclonal to Histone H1 (phospho) (1/100).Human neuroblastoma cell line SK-N-SH cultured on coverslips were fixed in 4% paraformaldehye and then stained with ab4270 (green). The DNA stained with DAPI is shown in red. (100x magnification).

Anti-Histone H1 (phospho S1 + T3) antibody - ab32979 from Abcam

Prices	$242.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-Histone H1 (phospho S1 + T3) antibody (ab32979) at 1 µg/mlLane 1 : Calf Thymus Histone Preparation at 0.5 µgLane 2 : Calf Thymus Histone Preparation at 0.5 µg with Human Histone H1 (phospho S1 + T3) peptide (ab36684) at 1 µg/mlLane 3 : Calf Thymus Histone Preparation at 0.5 µg with Human Histone H1 peptide (ab36686) at 1 µg/mlLane 4 : HeLa Histone Preparation Nuclear Lysate - Colcemid-treated at 2.5 µgLane 5 : HeLa Histone Preparation Nuclear Lysate - Colcemid-treated at 2.5 µg with Human Histone H1 (phospho S1 + T3) peptide (ab36684) at 1 µg/mlLane 6 : HeLa Histone Preparation Nuclear Lysate - Colcemid-treated at 2.5 µg with Human Histone H1 peptide (ab36686) at 1 µg/mlSecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

Anti-Histone H1 (phospho T146) antibody - ab3596 from Abcam

Prices	$401.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Rabbit polyclonal to Histone H1 phospho T146 (1/1000) against recombinant Histone H1, incubated with (control) untreated insect cell lysate (lanes 1, 3, 5, 7, 9, 11, 13) or insect cell lysate containing active cyclin E / CDK2 complexes (lanes 2, 4, 6, 8, 10, 12, 14).Using site-directed mutagenesis mutant Histone H1 proteins were made. Five phosphorylation cyclin/cdk phosphorylation concensus sites were mutated : T18, T146, T154, S172 and S187.Lanes 3-12 contain mutant histone H1 with only one wild-type cyclin/cdk phosphorylation concensus site (indicated in brakets).Lanes 13-14 contain mutant Histone H1 with all 5 sites mutated to Ala.Lanes 1-2 : wt Histone H1Lanes 3-4 : T146A, T154A, S172A, S187A (wt site at T18)Lanes 5-6 : T18A, T154A, S172A, S187A (wt site at T146)Lanes 7-8 : T18A, T146A, S172A, S187A (wt site at T154)Lanes 9-10 : T18A, T146A, T154A, S187A (wt site at S172)Lanes 11-12: T18A, T146A, T154A, S172A (wt site at S187)Lanes 13-14: T18A, T146A, T154A, S1

Anti-Histone H1 (phospho T17) antibody - ab61060 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Immunofluorescence analysis, using Histone H1 (phospho T17) antibody (ab61060) at 1/500 - 1/1000 dilution, of HuvEc cells with serum treatment (20%, 30mins)(left panel) or without serum treatment (right panel).

Anti-Histone H1.4 (phospho T18) antibody - ab3595 from Abcam

Prices	$380.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Rabbit polyclonal to Histone H1 phospho T18 (1/1000) against recombinant Histone H1, incubated with control lysate (lanes 1, 3, 5, 7, 9, 11, 13) or insect cell lysate containing active cyclin E / CDK2 complexes (lanes 2, 4, 6, 8, 10, 12, 14).Mutant Histone H1 proteins were made by site-directed mutagenesis of 5 phosphorylation cyclin/cdk phosphorylation concensus sites (T18, T146, T154, S172, S187). Lanes 3-12 contain mutant histone H1 with only one wild-type cyclin/cdk phosphorylation concensus site and lanes 13-14 containst mutant Histone H1 with all 5 sites mutated to Alanine.Lanes 1-2 : wt Histone H1Lanes 3-4 : T146A, T154A, S172A, S187A (wt site at T18)Lanes 5-6 : T18A, T154A, S172A, S187A (wt site at T146)Lanes 7-8 : T18A, T146A, S172A, S187A (wt site at T154)Lanes 9-10 : T18A, T146A, T154A, S187A (wt site at S172)Lanes 11-12: T18A, T146A, T154A, S172A (wt site at S187)Lanes 13-14: T18A, T146A, T154A, S172A, S187AAlejandro Contreras, Baylor College of Medicine

Anti-Histone H1.4 (tri methyl K25, phospho S26) antibody - ab33231 from Abcam

Prices	$242.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

This antibody gave a positive result in ELISA against the immunizing peptide (blue line, ab35913). For the following peptides it gave following results:the non-modified equivalent peptide (yellow line, ab35918) - weak result,H1.4 phospho S26 peptide (pink line, ab35917) - weak result.

Anti-Histone H1.4 antibody - ab116393 from Abcam

Prices	$370.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

IHC image of ab116393 staining Histone H1.4 in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab116393, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset). For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Histone H1.4 antibody - ab105522 from Abcam

Prices	$370.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

ICC/IF image of ab105522 stained SKNSH cells. The cells were4% formaldehyde fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab105522, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

Anti-HIST1H1E Antibody (aa1-30, FITC) - LS-C258508 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-HIST1H1E Antibody (aa1-30) - LS-C321965 from LifeSpan Bioscience

Prices	$345.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-HIST1H1E Antibody (aa1-30, Azide-free, HRP) - LS-C258509 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-HIST1H1E Antibody (aa1-30, PE) - LS-C258512 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-HIST1H1E Antibody (N-Terminus) - LS-C112173 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

HIST1H1E antibody LS-C112173 Western blot of ACHN lysate.

Anti-HIST1H1E Antibody (aa1-30) - LS-C166886 from LifeSpan Bioscience

Prices	$295.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

HIST1H1E Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human liver tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Anti-HIST1H1E Antibody (aa1-30, APC) - LS-C258505 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-HIST1H1E Antibody (aa1-30, AP) - LS-C258506 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-HIST1H1E Antibody (aa1-30, Biotin) - LS-C258507 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Histone H1.4 Antibody - NBP2-16847 from Novus Biologicals

Prices	$329.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: Histone H1.4 Antibody [NBP2-16847] - Sample (20 ug of cell lysate ) A: HeLa nucleus 12% SDS PAGE gel, diluted at 1:1000.

Histone H1.4 Antibody - NBP1-55150 from Novus Biologicals

Prices	$329.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: Histone H1.4 Antibody [NBP1-55150] - ACHN cell lysate, concentration 0.2-1 ug/ml.

Histone H1.4 Polyclonal Antibody - bs-10334R from Bioss

Prices	$269.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Histone H1.4(Thr18) Polyclonal Antibody - bs-3171R from Bioss

Prices	$289.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

$L1 mouse liver, L2 mouse cerebrum lysates probed (bs-3171R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted and observed band size: 24kDa.\\n$

Histone H1.4 (Thr146) Antibody - bs-3176R from Bioss

Prices	$289.00
Sizes	100 µl
Host	Rabbit

Histone H1.4 Polyclonal Antibody, ALEXA FLUOR® 350 Conjugated - bs-10334R-A350 from Bioss

Prices	$369.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

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