Anti-Histone H1.4 antibody
| Name | Anti-Histone H1.4 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab105522 |
| Prices | $370.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB |
| Species Reactivities | Human, Mouse, Rat, Rabbit, Guinea Pig, Bovine, Cat, Dog, Monkey |
| Antigen | Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (MSETAPAAPA APAPAEKTPV KKKARKSAGA AKRKASGPPV SELITKAVAA) of Human Histone H1 |
| Blocking Peptide | Histone H1.4 peptide |
| Description | Rabbit Polyclonal |
| Gene | HIST1H1E |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab105522 stained SKNSH cells. The cells were4% formaldehyde fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab105522, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM
Anti-Histone H1.4 antibody (ab105522) at 1 µg/ml + ACHN cell lysate at 10 µg