ICC/IF image of ab4633 stained human HEK 293 cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab4633, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Lanes 1 & 3 : Anti-Histone H2A (di methyl K95) antibody (ab4633) at 1/500 dilutionLanes 2 & 4 : Anti-Histone H2A (di methyl K95) antibody (ab4633) at 1/1000 dilutionLane 1 : 1ug of calf thymus Histone prepLane 2 : 1ug of calf thymus Histone prepLane 3 : 1ug of calf thymus Histone prep with Human Histone H2A (di methyl K95) peptide (ab10127) at 2 µgLane 4 : 1ug of calf thymus Histone prep with Human Histone H2A (di methyl K95) peptide (ab10127) at 2 µgSecondaryLanes 1 - 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 2000 Lane 4 : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution