HIST1H2AE

Gene Symbol	HIST1H2AE
Entrez Gene	3012
Alt Symbol	H2A.1, H2A.2, H2A/a, H2AFA
Species	Human
Gene Type	protein-coding
Description	histone cluster 1, H2ae
Other Description	H2A histone family, member A\|histone 1, H2ae\|histone H2A type 1-B/E\|histone H2A/a\|histone H2AE
Accessions	AAA63191 AAN59967 EAW55546 P04908 AK311824 BAG34766 BC093836 AAH93836 BC093862 AAH93862 EU446964 ABZ92493 NM_021052 NP_066390
Top Pathways	Systemic lupus erythematosus, Alcoholism

Anti-Histone H2A (acetyl K13) antibody - ab176427 from Abcam

Prices	$376.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-Histone H2A (acetyl K13) antibody (ab176427) at 1 µg/mlLane 1 : HeLa Sodium Butyrate-treated 0uM Control (Cell acid extract ) at 10 µgLane 2 : HeLa Sodium Butyrate-treated 10uM (Cell acid extract ) at 10 µgLane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µgLane 4 : Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µgSecondaryGoat Anti-Rabbit IgG H&L (Alexa Fluor® 790) (ab175781) at 1/10000 dilution

Anti-Histone H2A (acetyl K15) antibody - ab101447 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-Histone H2A (acetyl K15) antibody (ab101447) at 1/500 dilutionLane 1 : NIH/3T3 cell lysate, untreated Lane 2 : NIH/3T3 cell lysate, TSA-treated (to induce acetylation).

Anti-Histone H2A (acetyl K5) antibody - ab58548 from Abcam

Prices	$384.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

IHC image of ab58548 staining Histone H2A (acetyl K5) in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab58548, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Histone H2A (acetyl K9) antibody - ab47816 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-Histone H2A (acetyl K9) antibody (ab47816) at 1 µg/ml + HeLa whole cell lysate at 10 µgSecondaryDonkey Anti-Rabbit IgG H&L (HRP) (ab16284) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Histone H2A (acetyl K9) antibody [EPR17471] - ChIP Grade - ab177312 from Abcam

Prices	$347.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR17471

All lanes : Anti-Histone H2A (acetyl K9) antibody [EPR17471] - ChIP Grade (ab177312) at 1/1000 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) treated with 500 ng/ml Trichostatin A for 4 hours whole cell lysatesLane 2 : Untreated HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysatesLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-Histone H2A (di methyl K95) antibody - ab4633 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

ICC/IF image of ab4633 stained human HEK 293 cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab4633, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

Anti-Histone H2A (hydroxyl Y39) antibody [EPR17683] - ab177377 from Abcam

Prices	$99.00
Sizes	10 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR17683

All lanes : Anti-Histone H2A (hydroxyl Y39) antibody [EPR17683] (ab177377) at 1/2000 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysateLane 2 : NIH/3T3 (Mouse embyro fibroblast cells) cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-Histone H2A (mono methyl K118) antibody [EPR17488] - ab177387 from Abcam

Prices	$99.00
Sizes	10 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR17488

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Histone H2A (mono methyl K118) with ab177387 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:-ve control 1: ab177387 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

Anti-Histone H2A (mono methyl K125) antibody [EPR17493] - ab177392 from Abcam

Prices	$99.00
Sizes	10 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR17493

Anti-Histone H2A (mono methyl K127) antibody - ab12950 from Abcam

Prices	$242.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

ELISA eluate (red, blue) and flow through (green) using immunizing peptide (ab12952). Eluate (orange) and flow through (grey) using un-modified peptide (ab13202).This demonstrates that ab12950 specifically recognises mono-methyl K127 compared to the unmodified peptide.

Anti-Histone H2A (symmetric di methyl R29) antibody - ab129208 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-Histone H2A (symmetric di methyl R29) antibody (ab129208) at 1 µg/ml + Calf Thymus Histone Preparation Nuclear Lysate at 0.25 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Histone H2A (symmetric di methyl R3) antibody - ab22397 from Abcam

Prices	$242.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

IHC image of ab22397 staining Histone H2A (symmetric di methyl R3) in human kidney formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22397, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Histone H2A (symmetric di methyl R77) antibody - ab19923 from Abcam

Prices	$242.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

This antibody gave a positive result in ELISA against the immunizing peptide ab14952 (blue line) and a negative result against the non-modified peptide ab14953 (pink line).

Anti-Histone H2A antibody - ab18975 from Abcam

Prices	$378.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Anti-Histone H2A antibody (ab18975) at 1 µg/ml + HeLa cell lysate

Anti-Histone H2A antibody - ab93460 from Abcam

Prices	$359.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-Histone H2A antibody (ab93460) at 1/1000 dilutionLane 1 : A2058Lane 2 : A375Lane 3 : K562Lane 4 : HeLaLane 5 : MCF-7Lysates/proteins at 35 µg per lane.

Anti-Histone H2A antibody - ab198006 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-Histone H2A antibody (ab198006) at 1/250 dilutionLane 1 : K562 cell lysateLane 2 : Raji cell lysateLane 3 : HeLa cell lysateLane 4 : 293T cell lysateLysates/proteins at 40 µg per lane.

Anti-Histone H2A antibody - ab104075 from Abcam

Prices	$359.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-Histone H2A antibody (ab104075) at 1/100 dilution + HepG2 cell lysate at 35 µg

Anti-Histone H2A antibody - ab58550 from Abcam

Prices	$384.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

IHC image of ab58550 staining Histone H2A in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab58550, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset). For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Histone H2A antibody - ab116553 from Abcam

Prices	$376.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

IHC image of ab116553 staining Histone H2A in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab116553, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset). For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Histone H2A antibody [EPR17470] - C-terminal - ab177308 from Abcam

Prices	$347.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR17470

Anti-Histone H2A antibody [EPR17470] - C-terminal (ab177308) at 1/5000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-Histone H2A antibody - ChIP Grade - ab18255 from Abcam

Prices	$404.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 6µl of ab18255 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.

Anti-Histone H2A antibody - ChIP Grade - ab15653 from Abcam

Prices	$385.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-Histone H2A antibody - ChIP Grade (ab15653) at 0.5 µg/mlLane 1 : HeLa nuclear extractLane 2 : HeLa nuclear extract with Human Histone H2A peptide (ab15660) at 1 µg/ml

Anti-Histone H2A antibody - ChIP Grade - ab116677 from Abcam

Prices	$387.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 5µg of ab116677 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.

Anti-HIST1H2AE Antibody (aa60-109) - LS-C145651 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

HIST1H2AE antibody LS-C145651 Western blot of 8226 Cell lysate. Antibody concentration 1 ug/ml.

Anti-HIST1H2AE Antibody (N-Terminus) - LS-C187046 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Western blot of Human RPMI-8226. HIST1H2AE antibody dilution 1.0 ug/ml.

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