Anti-Histone H2A (phospho S129) antibody [EPR17588] - ChIP Grade

• Supplier: Abcam
• Catalog: ab181447
• Prices: $347.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR17588
• Applications: DB WB ChIP
• Species Reactivities: Yeast
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Saccharomyces cerevisiae Histone H2A aa 100 to the C-terminus (phospho S129)
• Description: Rabbit Monoclonal
• Gene: HTA1
• Conjugate: Unconjugated

Product images

Dot blot analysis of Histone H2A (phospho S129) peptide (Lane 1), and non-phospho peptide (Lane 2), labeled using ab181447 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.Blocking/Dilution buffer: 5% NFDM/TBST.Exposure time : 3 minutes

All lanes : Anti-Histone H2A (phospho S129) antibody [EPR17588] - ChIP Grade (ab181447) at 1/5000 dilutionLane 1 : Saccharomyces cerevisiae treated with 0.2% Methyl methanesulfonate for 1 hour lysateLane 2 : Untreated Saccharomyces cerevisiae whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Chromatin was prepared from Saccharomyces cerevisiae cells according to the Abcam X-ChIP protocol. Saccharomyces cerevisiae cells were treated with MMS at 2mg/ml for 1 h. Treated and non-treated Saccharomyces cerevisiae cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab181447 (blue), and 20µl of Anti rabbit IgG sepharose beads. 2μg of rabbit normal IgG was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).