Anti-Histone H2A (symmetric di methyl R77) antibody
| Name | Anti-Histone H2A (symmetric di methyl R77) antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab19923 |
| Prices | $242.00 |
| Sizes | 200 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Human Histone H2A |
| Description | Rabbit Polyclonal |
| Gene | HIST1H2AE |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
This antibody gave a positive result in ELISA against the immunizing peptide ab14952 (blue line) and a negative result against the non-modified peptide ab14953 (pink line).
ICC/IF image of ab19923 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab19923, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).