Anti-Histone H2A.Z antibody - ChIP Grade
| Name | Anti-Histone H2A.Z antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab4174 |
| Prices | $403.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF ChIP IP ChIPseq WB |
| Species Reactivities | Mouse, Rat, Rabbit, Chicken, Bovine, Human, Arabidopsis thaliana, Zebrafish, Sheep, Xenopus |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human Histone H2A |
| Blocking Peptide | Human Histone H2A.Z peptide |
| Description | Rabbit Polyclonal |
| Gene | H2AFZ |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
The staining has been done on mouse embryonic cells. The fixation is 2% PFA, and permeabilization is PBS 0.5% triton BSA. The dilution used was 1 in 100 (but it could be used at 1/200 to1/300)Red = H2A.Z Blue = toto3 for the DNA
Performed under reducing conditions.
Performed under reducing conditions.
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 2 µg of ab4174 (blue), and 20 µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
This image was kindly supplied as part of the review submitted by Dr Geza Fejes-Toth.
developed using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab4174 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4174, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
developed using the ECL techniquePerformed under reducing conditions.
Product References
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